RRC ID 65602
著者 Kuwano T, Izumi H, Aslam MR, Igarashi Y, Bilal M, Nishimura A, Watanabe Y, Nawaz A, Kado T, Ikuta K, Yamamoto S, Sasahara M, Fujisaka S, Yagi K, Mori H, Tobe K.
タイトル Generation and characterization of a Meflin-CreERT2 transgenic line for lineage tracing in white adipose tissue.
ジャーナル PLoS One
Abstract Meflin (Islr) expression has gained attention as a marker for mesenchymal stem cells, but its function remains largely unexplored. Here, we report the generation of Meflin-CreERT2 mice with CreERT2 inserted under the Meflin gene promoter to label Meflin-expressing cells genetically, thereby enabling their lineages to be traced. We found that in adult mice, Meflin-expressing lineage cells were present in adipose tissue stroma and had differentiated into mature adipocytes. These cells constituted Crown-like structures in the adipose tissue of mice after high-fat diet loading. Cold stimulation led to the differentiation of Meflin-expressing lineage cells into beige adipocytes. Thus, the Meflin-CreERT2 mouse line is a useful new tool for visualizing and tracking the lineage of Meflin-expressing cells.
巻・号 16(3)
ページ e0248267
公開日 2021-1-1
DOI 10.1371/journal.pone.0248267
PII PONE-D-20-35143
PMID 33760832
PMC PMC7990287
MeSH Adipose Tissue, White* / cytology Adipose Tissue, White* / metabolism Animals Cell Differentiation Cell Lineage Gene Expression Immunoglobulins* / genetics Immunoglobulins* / metabolism Mesenchymal Stem Cells / cytology* Mice Mice, Inbred C57BL Mice, Transgenic*
IF 2.74
リソース情報
遺伝子材料 B6N Mouse BAC clone (RDB07573) B6Ng01-165L10P