論文 - 詳細
| RRC ID | 65618 |
|---|---|
| 著者 | Tsujii M, Kera K, Hamamoto S, Kuromori T, Shikanai T, Uozumi N. |
| タイトル | Evidence for potassium transport activity of Arabidopsis KEA1-KEA6. |
| ジャーナル | Sci Rep |
| Abstract |
Arabidopsis thaliana contains the putative K+ efflux transporters KEA1-KEA6, similar to KefB and KefC of Escherichia coli. KEA1-KEA3 are involved in the regulation of photosynthetic electron transport and chloroplast development. KEA4-KEA6 mediate pH regulation of the endomembrane network during salinity stress. However, the ion transport activities of KEA1-KEA6 have not been directly characterized. In this study, we used an E. coli expression system to examine KEA activity. KEA1-KEA3 and KEA5 showed bi-directional K+ transport activity, whereas KEA4 and KEA6 functioned as a K+ uptake system. The thylakoid membrane-localized Na+/H+ antiporter NhaS3 from the model cyanobacterium Synechocystis is the closest homolog of KEA3. Changing the putative Na+/H+ selective site of KEA3 (Gln-Asp) to that of NhaS3 (Asp-Asp) did not alter the ion selectivity without loss of K+ transport activity. The first residue in the conserved motif was not a determinant for K+ or Na+ selectivity. Deletion of the possible nucleotide-binding KTN domain from KEA3 lowered K+ transport activity, indicating that the KTN domain was important for this function. The KEA3-G422R mutation discovered in the Arabidopsis dpgr mutant increased K+ transport activity, consistent with the mutant phenotype. These results indicate that Arabidopsis KEA1-KEA6 act as K+ transport systems, and support the interpretation that KEA3 promotes dissipation of ΔpH in the thylakoid membrane. |
| 巻・号 | 9(1) |
| ページ | 10040 |
| 公開日 | 2019-7-11 |
| DOI | 10.1038/s41598-019-46463-7 |
| PII | 10.1038/s41598-019-46463-7 |
| PMID | 31296940 |
| PMC | PMC6624313 |
| MeSH | Antiporters / metabolism Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Ion Transport / physiology Potassium / metabolism* Potassium-Hydrogen Antiporters / genetics Potassium-Hydrogen Antiporters / metabolism* Protein Isoforms / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda04049 |