| RRC ID |
65621
|
| 著者 |
Kimura K, Ramirez K, Nguyen TAV, Yamashiro Y, Sada A, Yanagisawa H.
|
| タイトル |
Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The maladaptive remodeling of vessel walls with neointima formation is a common feature of proliferative vascular diseases. It has been proposed that neointima formation is caused by the dedifferentiation of mature smooth muscle cells (SMCs). Recent evidence suggests that adventitial cells also participate in neointima formation; however, their cellular dynamics are not fully understood. In this study, we utilized a lineage tracing model of platelet-derived growth factor receptor alpha (PDGFRa) cells and examined cellular behavior during homeostasis and injury response. PDGFRa marked adventitial cells that were largely positive for Sca1 and a portion of medial SMCs, and both cell types were maintained for 2 years. Upon carotid artery ligation, PDGFRa-positive (+) cells were slowly recruited to the neointima and exhibited an immature SMC phenotype. In contrast, in a more severe wire denudation injury, PDGFRa+ cells were recruited to the neointima within 14 days and fully differentiated into SMCs. Under pressure overload induced by transverse aortic constriction, PDGFRa+ cells developed marked adventitial fibrosis. Taken together, our observations suggest that PDGFRa+ cells serve as a reservoir of adventitial cells and a subset of medial SMCs and underscore their context-dependent response to vascular injuries.
|
| 巻・号 |
11(1)
|
| ページ |
8683
|
| 公開日 |
2021-4-21
|
| DOI |
10.1038/s41598-021-88126-6
|
| PII |
10.1038/s41598-021-88126-6
|
| PMID |
33883668
|
| PMC |
PMC8060414
|
| MeSH |
Adventitia / metabolism
Animals
Blood Vessels / injuries*
Blood Vessels / metabolism
Blood Vessels / pathology
Blood Vessels / physiology
Cell Proliferation
Homeostasis
Male
Mice
Mice, Transgenic
Neointima / metabolism
Receptor, Platelet-Derived Growth Factor alpha / metabolism*
|
| IF |
3.998
|
| リソース情報 |
| 実験動物マウス |
RBRC09616 |
