RRC ID 65621
Author Kimura K, Ramirez K, Nguyen TAV, Yamashiro Y, Sada A, Yanagisawa H.
Title Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels.
Journal Sci Rep
Abstract The maladaptive remodeling of vessel walls with neointima formation is a common feature of proliferative vascular diseases. It has been proposed that neointima formation is caused by the dedifferentiation of mature smooth muscle cells (SMCs). Recent evidence suggests that adventitial cells also participate in neointima formation; however, their cellular dynamics are not fully understood. In this study, we utilized a lineage tracing model of platelet-derived growth factor receptor alpha (PDGFRa) cells and examined cellular behavior during homeostasis and injury response. PDGFRa marked adventitial cells that were largely positive for Sca1 and a portion of medial SMCs, and both cell types were maintained for 2 years. Upon carotid artery ligation, PDGFRa-positive (+) cells were slowly recruited to the neointima and exhibited an immature SMC phenotype. In contrast, in a more severe wire denudation injury, PDGFRa+ cells were recruited to the neointima within 14 days and fully differentiated into SMCs. Under pressure overload induced by transverse aortic constriction, PDGFRa+ cells developed marked adventitial fibrosis. Taken together, our observations suggest that PDGFRa+ cells serve as a reservoir of adventitial cells and a subset of medial SMCs and underscore their context-dependent response to vascular injuries.
Volume 11(1)
Pages 8683
Published 2021-4-21
DOI 10.1038/s41598-021-88126-6
PII 10.1038/s41598-021-88126-6
PMID 33883668
PMC PMC8060414
MeSH Adventitia / metabolism Animals Blood Vessels / injuries* Blood Vessels / metabolism Blood Vessels / pathology Blood Vessels / physiology Cell Proliferation Homeostasis Male Mice Mice, Transgenic Neointima / metabolism Receptor, Platelet-Derived Growth Factor alpha / metabolism*
IF 3.998
Mice RBRC09616