| RRC ID |
65625
|
| 著者 |
Chiu YH, Wu YW, Hung JI, Chen MC.
|
| タイトル |
Epigallocatechin gallate/L-ascorbic acid-loaded poly-γ-glutamate microneedles with antioxidant, anti-inflammatory, and immunomodulatory effects for the treatment of atopic dermatitis.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
Epigallocatechin gallate (EGCG) is a potential therapeutic agent for treatment of atopic dermatitis (AD) due to its antioxidant and anti-inflammatory activities. However, inherent instability of EGCG greatly limits its bioavailability and clinical efficacy. In this study, we developed a poly-γ-glutamate (γ-PGA)-based microneedle (MN) formulation capable of maintaining EGCG's stability and efficiently delivering EGCG into the skin to ameliorate AD symptoms. The γ-PGA MN can not only protect EGCG from oxidation, but also serve as an immunomodulator to downregulate T helper type 2 (Th2)-type immune responses. Encapsulation of EGCG into the γ-PGA MN and utilization of L-ascorbic acid (AA) as a stabilizer preserved 95% of its structural stability and retained 93% of its initial antioxidant activity after 4 weeks of storage. Once-weekly administration of EGCG/AA-loaded MNs to an Nc/Nga mouse model of AD for 4 weeks significantly ameliorated skin lesions and epidermal hyperplasia by reducing serum IgE (from 12156 ± 1344 to 5555 ± 1362 ng/mL) and histamine levels (from 81 ± 18 to 40 ± 5 pg/mL) and inhibiting IFN-γ (from 0.10 ± 0.01 to 0.01 pg/mg total protein) and Th2-type cytokine production, when compared to the AD (no treatment) group (p < 0.05). Notably, once-weekly MN therapy was at least as effective as the daily topical application of an EGCG + AA solution but markedly reduced the administration frequency and required dose. These results show that EGCG/AA-loaded γ-PGA MNs may be a convenient and promising therapeutic option for AD treatment.
|
| 巻・号 |
130
|
| ページ |
223-233
|
| 公開日 |
2021-8-1
|
| DOI |
10.1016/j.actbio.2021.05.032
|
| PII |
S1742-7061(21)00342-1
|
| PMID |
34087444
|
| MeSH |
Animals
Anti-Inflammatory Agents / pharmacology
Anti-Inflammatory Agents / therapeutic use
Antioxidants / therapeutic use
Ascorbic Acid / pharmacology
Catechin / analogs & derivatives
Cytokines
Dermatitis, Atopic* / drug therapy
Humans
Immunity
Mice
Polyglutamic Acid / analogs & derivatives
Skin
|
| IF |
7.242
|
| リソース情報 |
| 実験動物マウス |
RBRC01059 |
