論文 - 詳細
| RRC ID | 65636 |
|---|---|
| 著者 | Hincha DK, Zuther E, Popova AV. |
| タイトル | Stabilization of Dry Sucrose Glasses by Four LEA_4 Proteins from Arabidopsis thaliana. |
| ジャーナル | Biomolecules |
| Abstract |
Cells of many organisms and organs can withstand an (almost) total water loss (anhydrobiosis). Sugars play an essential role in desiccation tolerance due to their glass formation ability during dehydration. In addition, intrinsically disordered LEA proteins contribute to cellular survival under such conditions. One possible mechanism of LEA protein function is the stabilization of sugar glasses. However, little is known about the underlying mechanisms. Here we used FTIR spectroscopy to investigate sucrose (Suc) glass stability dried from water or from two buffer components in the presence of four recombinant LEA and globular reference proteins. Buffer ions influenced the strength of the Suc glass in the order Suc < Suc/Tris < Suc/NaP. LEA proteins strengthened the sugar H-bonded network and the molecular structure in the glassy state. The position of νOH peak and the wavenumber-temperature coefficient (WTCg) provided similar information about the H-bonded network. Protein aggregation of LEA proteins was reduced in the desiccation-induced Suc glassy state. Detailed knowledge about the role of LEA proteins in the stabilization of dry sugar glasses yields information about their role in anhydrobiosis. This may open the possibility to use such proteins in biotechnical applications requiring dry storage of biologicals such as proteins, cells or tissues. |
| 巻・号 | 11(5) |
| 公開日 | 2021-4-21 |
| DOI | 10.3390/biom11050615 |
| PII | biom11050615 |
| PMID | 33919135 |
| PMC | PMC8143093 |
| MeSH | Amino Acid Sequence / genetics Arabidopsis / enzymology Arabidopsis / metabolism* Arabidopsis Proteins / metabolism Carbohydrates / chemistry Desiccation Intrinsically Disordered Proteins / chemistry Plant Proteins / chemistry* Plant Proteins / metabolism Spectroscopy, Fourier Transform Infrared / methods Sucrose / chemistry* Sucrose / metabolism Sugars / chemistry Sugars / metabolism Water / metabolism |
| IF | 4.082 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda05667 |