RRC ID 65657
著者 Okita Y, Zheng L, Kawanishi K, Miyoshi H, Yanagihara K, Kato M.
タイトル Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation.
ジャーナル Genes Cells
Abstract Three-dimensional (3D) culturing mimics the heterogeneous cellular conditions of the in vivo tumor microenvironment compared to 2D monolayer-cultured cells and 3D cultures of established cancer cell lines (sphere culture) or patient-derived cancer cells (organoid culture) are frequently used for cancer research or drug screening and evaluation. To establish more cost and time-efficient 3D culture methods for cancer cell lines, we supplemented sphere culture medium with polyvinyl alcohol (PVA) and found that 3D sphere cultures of breast and pancreatic cancer cell lines were significantly increased. Mechanistically, we found that PVA prevented cell death and promoted cellular proliferation while maintaining levels of stemness-related gene expression. Furthermore, we showed that polyvinyl formal resin (PVF) 3D scaffolds made by cross-linked PVA can function in serum-free, long-term 3D cultures to support maintenance of sphere- or tumor-like cell masses for diverse cancer cell types. Taken together, we demonstrate the effectiveness of PVA and PVF in human cancer cell line culture protocols.
巻・号 26(5)
ページ 336-343
公開日 2021-5-1
DOI 10.1111/gtc.12843
PMID 33638919
MeSH Apoptosis / drug effects* Cell Line, Tumor Cell Proliferation / drug effects Gene Expression Regulation, Neoplastic / drug effects Humans Neoplastic Stem Cells / drug effects Neoplastic Stem Cells / metabolism Polyvinyl Alcohol / pharmacology* Spheroids, Cellular / cytology* Spheroids, Cellular / drug effects
IF 1.655
リソース情報
ヒト・動物細胞 RERF-LC-AI(RCB0444)