RRC ID |
65657
|
著者 |
Okita Y, Zheng L, Kawanishi K, Miyoshi H, Yanagihara K, Kato M.
|
タイトル |
Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation.
|
ジャーナル |
Genes Cells
|
Abstract |
Three-dimensional (3D) culturing mimics the heterogeneous cellular conditions of the in vivo tumor microenvironment compared to 2D monolayer-cultured cells and 3D cultures of established cancer cell lines (sphere culture) or patient-derived cancer cells (organoid culture) are frequently used for cancer research or drug screening and evaluation. To establish more cost and time-efficient 3D culture methods for cancer cell lines, we supplemented sphere culture medium with polyvinyl alcohol (PVA) and found that 3D sphere cultures of breast and pancreatic cancer cell lines were significantly increased. Mechanistically, we found that PVA prevented cell death and promoted cellular proliferation while maintaining levels of stemness-related gene expression. Furthermore, we showed that polyvinyl formal resin (PVF) 3D scaffolds made by cross-linked PVA can function in serum-free, long-term 3D cultures to support maintenance of sphere- or tumor-like cell masses for diverse cancer cell types. Taken together, we demonstrate the effectiveness of PVA and PVF in human cancer cell line culture protocols.
|
巻・号 |
26(5)
|
ページ |
336-343
|
公開日 |
2021-5-1
|
DOI |
10.1111/gtc.12843
|
PMID |
33638919
|
MeSH |
Apoptosis / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Humans
Neoplastic Stem Cells / drug effects
Neoplastic Stem Cells / metabolism
Polyvinyl Alcohol / pharmacology*
Spheroids, Cellular / cytology*
Spheroids, Cellular / drug effects
|
IF |
1.655
|
リソース情報 |
ヒト・動物細胞 |
RERF-LC-AI(RCB0444) |