| RRC ID |
65661
|
| Author |
Wei M, Li S, Yang Z, Cheng C, Li T, Le W.
|
| Title |
Tetrahedral DNA nanostructures functionalized by multivalent microRNA132 antisense oligonucleotides promote the differentiation of mouse embryonic stem cells into dopaminergic neurons.
|
| Journal |
Nanomedicine
|
| Abstract |
MicroRNA132 (miR132) negatively regulates the differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DAergic) neurons; in contrast, antisense oligonucleotide against miR132 (miR132-ASO) effectively blocks the activity of endogenous miR132 and thereafter promotes the differentiation of DAergic neurons. However, it is difficult for miR132-ASO to enter cells without a suitable delivery system. Tetrahedral DNA nanostructures (TDNs), as a new type of DNA-based nanocarrier, have great potential in biomedical applications and even have been reported to promote stem cell differentiation. In this study, we developed functional multivalent DNA nanostructures by appending miR132-ASO motifs to three-dimensional TDNs (miR132-ASO-TDNs). Our data clearly revealed that miR132-ASO-TDNs exposure can promote the differentiation of ESCs into DAergic neurons as well as elevate DA release from differentiated DAergic neurons. MiR132-ASO-TDNs could serve as a novel biofunctional nanomaterial to improve the efficiency of DAergic neurons differentiation. Our findings may also provide a new approach for stem cell therapy against neurodegenerative diseases.
|
| Volume |
34
|
| Pages |
102375
|
| Published |
2021-6-1
|
| DOI |
10.1016/j.nano.2021.102375
|
| PII |
S1549-9634(21)00018-6
|
| PMID |
33617970
|
| MeSH |
Animals
Cell Differentiation / drug effects*
Cell Line
DNA / chemistry*
Dopaminergic Neurons / cytology
Dopaminergic Neurons / drug effects*
Mice
MicroRNAs / genetics*
Mouse Embryonic Stem Cells / cytology
Mouse Embryonic Stem Cells / drug effects*
Nucleic Acid Conformation*
RNA, Antisense / pharmacology*
|
| IF |
4.3
|
| Resource |
| Human and Animal Cells |
MC3T3-G2/PA6(RCB1127) |
