Reference - Detail
| RRC ID | 65689 |
|---|---|
| Author | Sano K, Miki M, Tanaka T, Munemura M, Munekane M, Yamasaki T, Mukai T. |
| Title | Electrostatically self-assembled gold nanorods with chondroitin sulfate for targeted photothermal therapy for melanoma. |
| Journal | Photodiagnosis Photodyn Ther |
| Abstract |
BACKGROUND:The application of gold nanorods (GNRs) in photothermal therapy is a promising avenue for cancer treatment. The aim of this study was to develop a GNR-based targeted photothermal therapy for melanoma. METHODS:We utilized the electrostatic interaction between cationic GNRs and an anionic polymer chondroitin sulfate A (CSA), which has an affinity for binding to melanoma cells, to construct an anionic binary GNR-CSA complex (GNR-CS) at an optimal theoretical charge ratio of the trimethylammonium groups of GNR: carboxyl and sulfate groups of CSA = 1:2.5. The cytotoxicity to normal cells and erythrocyte agglutination activity of GNR-CS were evaluated. After the cellular uptake of GNR-CS by melanoma cells (B16-F10) was investigated, the photothermal performance of GNR-CS against B16-F10 cells was evaluated in vitro. RESULTS:The particle size and zeta potential of GNR-CS were approximately 35 nm and -20 mV, respectively. GNR-CS showed little cytotoxicity to normal cells and low erythrocyte agglutination activity, indicating good biocompatibility. Compared with negatively-charged GNR, GNR-CS was highly taken up by B16-F10 cells even if it was negatively charged. Cellular uptake was significantly suppressed upon treatment with excess CSA, suggesting the involvement of a CSA-specific uptake pathway. Furthermore, irradiation of the GNR-CS solution with near-infrared (NIR) light increased its temperature in light-intensity and GNR-concentration dependent manners. GNR-CS exhibited significant and GNR-dose dependent cytotoxicity in melanoma cells in combination with NIR light irradiation. CONCLUSION:GNRs coated with CSA have the potential as a medicine in targeted photothermal therapy for melanoma. |
| Volume | 35 |
| Pages | 102402 |
| Published | 2021-9-1 |
| DOI | 10.1016/j.pdpdt.2021.102402 |
| PII | S1572-1000(21)00229-5 |
| PMID | 34144259 |
| MeSH | Cell Line, Tumor Chondroitin Sulfates Gold Humans Melanoma* / drug therapy Nanotubes* Photochemotherapy* / methods Photosensitizing Agents / pharmacology Phototherapy Photothermal Therapy |
| IF | 2.894 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |