論文 - 詳細
RRC ID | 65695 |
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著者 | Yamashita M, Kumasawa K, Miyake T, Nakamura H, Kimura T. |
タイトル | Soluble Flt-1 Has Cytotoxic Effects on BeWo Choriocarcinoma Cells. |
ジャーナル | Reprod Sci |
Abstract |
BACKGROUND:Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis and is overexpressed in many kinds of malignant tumors. Soluble VEGF receptor 1 (sVEGFR-1/ soluble fms-like tyrosine kinase 1 [sFlt-1]) plays a role as an inhibitor of VEGF, and an antitumor effect has been shown in several studies using sFlt-1. Recently, in addition to its antiangiogenic effect, it was reported that sFlt-1 has direct cytotoxicity. MATERIALS AND METHODS:Transfection of sFlt-1 plasmid DNA was performed in the BeWo choriocarcinoma cell line. Overexpression of sFlt-1 in BeWo cells was confirmed by ELISA. In order to evaluate cell proliferation, cell counting and BrdU uptake assay were performed. Cytotoxicity was tested by LDH assay. TdT-Mediated dUTP Nick end Labeling (TUNEL) staining and quantitative analysis of caspase-cleaved keratin 18 (ccK18) level were done to evaluate cell apoptosis. RESULTS:The cell number was significantly less, and the ratio of cytotoxicity was significantly higher in sFlt-1 group compared to the control group. TUNEL staining and ccK18 level suggested nonapoptotic cell death. CONCLUSION:Soluble Flt-1 showed a cytotoxic effect on BeWo cells. Our results suggest that sFLT-1 could be therapeutic for malignant tumors. |
巻・号 | 25(6) |
ページ | 830-836 |
公開日 | 2018-6-1 |
DOI | 10.1177/1933719117698575 |
PMID | 28322131 |
MeSH | Antineoplastic Agents / pharmacology* Apoptosis / drug effects Cell Line, Tumor Cell Proliferation Choriocarcinoma / drug therapy Choriocarcinoma / enzymology* Humans L-Lactate Dehydrogenase / analysis Membrane Proteins / analysis Transfection Vascular Endothelial Growth Factor A / analysis Vascular Endothelial Growth Factor Receptor-1 / metabolism Vascular Endothelial Growth Factor Receptor-1 / pharmacology* |
IF | 2.616 |
リソース情報 | |
ヒト・動物細胞 | BeWo |