RRC ID 65695
著者 Yamashita M, Kumasawa K, Miyake T, Nakamura H, Kimura T.
タイトル Soluble Flt-1 Has Cytotoxic Effects on BeWo Choriocarcinoma Cells.
ジャーナル Reprod Sci
Abstract BACKGROUND:Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis and is overexpressed in many kinds of malignant tumors. Soluble VEGF receptor 1 (sVEGFR-1/ soluble fms-like tyrosine kinase 1 [sFlt-1]) plays a role as an inhibitor of VEGF, and an antitumor effect has been shown in several studies using sFlt-1. Recently, in addition to its antiangiogenic effect, it was reported that sFlt-1 has direct cytotoxicity.
MATERIALS AND METHODS:Transfection of sFlt-1 plasmid DNA was performed in the BeWo choriocarcinoma cell line. Overexpression of sFlt-1 in BeWo cells was confirmed by ELISA. In order to evaluate cell proliferation, cell counting and BrdU uptake assay were performed. Cytotoxicity was tested by LDH assay. TdT-Mediated dUTP Nick end Labeling (TUNEL) staining and quantitative analysis of caspase-cleaved keratin 18 (ccK18) level were done to evaluate cell apoptosis.
RESULTS:The cell number was significantly less, and the ratio of cytotoxicity was significantly higher in sFlt-1 group compared to the control group. TUNEL staining and ccK18 level suggested nonapoptotic cell death.
CONCLUSION:Soluble Flt-1 showed a cytotoxic effect on BeWo cells. Our results suggest that sFLT-1 could be therapeutic for malignant tumors.
巻・号 25(6)
ページ 830-836
公開日 2018-6-1
DOI 10.1177/1933719117698575
PMID 28322131
MeSH Antineoplastic Agents / pharmacology* Apoptosis / drug effects Cell Line, Tumor Cell Proliferation Choriocarcinoma / drug therapy Choriocarcinoma / enzymology* Humans L-Lactate Dehydrogenase / analysis Membrane Proteins / analysis Transfection Vascular Endothelial Growth Factor A / analysis Vascular Endothelial Growth Factor Receptor-1 / metabolism Vascular Endothelial Growth Factor Receptor-1 / pharmacology*
IF 2.616
リソース情報
ヒト・動物細胞 BeWo