RRC ID 65697
Author Kawakubo N, Tanaka S, Kinoshita Y, Tajiri T, Yonemitsu Y, Taguchi T.
Title Sequential actions of immune effector cells induced by viral activation of dendritic cells to eliminate murine neuroblastoma.
Journal J Pediatr Surg
Abstract PURPOSE:In preclinical trails, we reported the antitumor effect of dendritic cells activated with Sendai virus (rSeV/DC) combined with γ-irradiation against neuroblastoma. However, what kind of effector cells for the combined therapy were used to show the antitumor effect was unclear. In this study, we performed radiation and rSeV/DC therapy in vivo and examined the effector cells involved.
METHODS:Dendritic cells were cultured from bone marrow cells, activated with SeV and administered intratumorally at 106 weekly for 3weeks. Radiation was administered at 4Gy/time × 3 times. During the treatment, CD4+ and CD8+ cells and natural killer (NK) cells were removed by antibodies.
RESULTS:Complete remission of neuroblastoma was observed in 62.5% of individuals in the combined therapy group. By depleting the effector cells using antibodies, the tumor increased in size from an early stage of treatment in the CD4+ and NK cell-depleted group. In contrast, the tumor increased in size in the late stage of treatment in the CD8+ cell-depleted group.
CONCLUSION:The combination of radiation and rSeV/DC therapy induces different effector cells, depending on the time point during treatment.
LEVEL OF EVIDENCE:V.
Volume 53(8)
Pages 1615-1620
Published 2018-8-1
DOI 10.1016/j.jpedsurg.2017.08.019
PII S0022-3468(17)30515-8
PMID 28941928
MeSH Animals Bone Marrow Cells / pathology Dendritic Cells / immunology* Humans Killer Cells, Natural Lymphocyte Activation / immunology* Mice Neuroblastoma / immunology* Neuroblastoma / pathology Sendai virus Virus Activation / immunology*
IF 1.919
Resource
Human and Animal Cells C-1300(RCB0283) MuSS(RCB1378)