論文 - 詳細
RRC ID | 65697 |
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著者 | Kawakubo N, Tanaka S, Kinoshita Y, Tajiri T, Yonemitsu Y, Taguchi T. |
タイトル | Sequential actions of immune effector cells induced by viral activation of dendritic cells to eliminate murine neuroblastoma. |
ジャーナル | J Pediatr Surg |
Abstract |
PURPOSE:In preclinical trails, we reported the antitumor effect of dendritic cells activated with Sendai virus (rSeV/DC) combined with γ-irradiation against neuroblastoma. However, what kind of effector cells for the combined therapy were used to show the antitumor effect was unclear. In this study, we performed radiation and rSeV/DC therapy in vivo and examined the effector cells involved. METHODS:Dendritic cells were cultured from bone marrow cells, activated with SeV and administered intratumorally at 106 weekly for 3weeks. Radiation was administered at 4Gy/time × 3 times. During the treatment, CD4+ and CD8+ cells and natural killer (NK) cells were removed by antibodies. RESULTS:Complete remission of neuroblastoma was observed in 62.5% of individuals in the combined therapy group. By depleting the effector cells using antibodies, the tumor increased in size from an early stage of treatment in the CD4+ and NK cell-depleted group. In contrast, the tumor increased in size in the late stage of treatment in the CD8+ cell-depleted group. CONCLUSION:The combination of radiation and rSeV/DC therapy induces different effector cells, depending on the time point during treatment. LEVEL OF EVIDENCE:V. |
巻・号 | 53(8) |
ページ | 1615-1620 |
公開日 | 2018-8-1 |
DOI | 10.1016/j.jpedsurg.2017.08.019 |
PII | S0022-3468(17)30515-8 |
PMID | 28941928 |
MeSH | Animals Bone Marrow Cells / pathology Dendritic Cells / immunology* Humans Killer Cells, Natural Lymphocyte Activation / immunology* Mice Neuroblastoma / immunology* Neuroblastoma / pathology Sendai virus Virus Activation / immunology* |
IF | 1.919 |
リソース情報 | |
ヒト・動物細胞 | C-1300(RCB0283) MuSS(RCB1378) |