論文 - 詳細
| RRC ID | 65703 |
|---|---|
| 著者 | Matsubara T, Kokabu S, Nakatomi C, Kinbara M, Maeda T, Yoshizawa M, Yasuda H, Takano-Yamamoto T, Baron R, Jimi E. |
| タイトル | The Actin-Binding Protein PPP1r18 Regulates Maturation, Actin Organization, and Bone Resorption Activity of Osteoclasts. |
| ジャーナル | Mol Cell Biol |
| Abstract |
Osteoclasts resorb bone by attaching on the bone matrix and forming a sealing zone. In Src-deficient mice, osteoclasts cannot form the actin ring, a characteristic actin structure that seals the resorbed area, and resorb hardly any bone as a result. However, the molecular mechanism underlying the role of Src in the regulation and organization of the actin ring is still unclear. We identified an actin-regulatory protein, protein phosphatase 1 regulatory subunit 18 (PPP1r18), as an Src-binding protein in an Src-, Yes-, and Fyn-deficient fibroblast (SYF) cell line overexpressing a constitutively active form of Src. PPP1r18 was localized in the nucleus and actin ring. PPP1r18 overexpression in osteoclasts inhibited terminal differentiation, actin ring formation, and bone-resorbing activity. A mutation of the protein phosphatase 1 (PP1)-binding domain of PPP1r18 rescued these phenotypes. In contrast, PPP1r18 knockdown promoted terminal differentiation and actin ring formation. In summary, we showed that PPP1r18 likely plays a role in podosome organization and bone resorption. |
| 巻・号 | 38(4) |
| 公開日 | 2018-2-15 |
| DOI | 10.1128/MCB.00425-17 |
| PII | MCB.00425-17 |
| PMID | 29158294 |
| PMC | PMC5789032 |
| MeSH | Actins / metabolism* Animals Bone Resorption / metabolism* Cell Adhesion / physiology Cell Differentiation / physiology Cell Line Dopamine and cAMP-Regulated Phosphoprotein 32 / metabolism* Humans Male Mice Mice, Transgenic Osteoclasts / cytology* Osteoclasts / metabolism* Protein Binding Protein Phosphatase 1 / metabolism Protein-Tyrosine Kinases / metabolism Receptors, Neuropeptide Y / metabolism src-Family Kinases / metabolism |
| IF | 3.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |