RRC ID 65738
著者 Hirano S, Zhou Q, Furuyama A, Kanno S.
タイトル Differential Regulation of IL-1β and IL-6 Release in Murine Macrophages.
ジャーナル Inflammation
Abstract Asbestos and silica (exogenous danger) and adenosine triphosphate (ATP, endogenous danger-signaling molecule) synergistically increase IL-1β release from endotoxin-primed macrophage, which is mediated by NOD-like receptor protein 3 (NLRP3) inflammasome. However, the conversion of pro-IL-1β to its active form seems to depend on the macrophage cell types. In the present study, bone marrow-derived macrophages (BMM) and three murine macrophage cell lines, J774.1, J774A.1, and RAW264.7 were exposed to ATP or fibrous titanium dioxide (FTiO2) in the presence or absence of lipopolysaccharide (LPS), and the concentrations of IL-1β and IL-6 in both cell lysates and in the culture media were measured by immunoblotting to differentiate active form of IL-1β from pro-IL-1β. IL-1β release was synergistically increased when the cells were exposed to both LPS and ATP or FTiO2, while IL-6 was readily released by LPS alone. IL-1β released into the culture medium was pro-IL-1β in J774.1 and RAW264.7, and most of the pro-IL-1β remained inside the cells. In contrast, the active form of IL-1β was released together with pro-IL-1β from J774A.1 and BMM after the co-stimulation. J774A.1 and BMM express apoptosis-associated speck-like protein contains a carboxyl-terminal CARD (ASC) while J774.1 and RAW264.7 do not or only faintly express ASC, and accordingly, caspase-1, which converts pro-IL-1β to its active form, is activated only in J774A.1 and BMM. Collectively, the canonical inflammasome pathway is not activated in J774.1 and RAW264.7, and the apparent synergistical increase of IL-1β in the culture medium mostly reflects the leakage of pro-IL-1β from these cells.
巻・号 40(6)
ページ 1933-1943
公開日 2017-12-1
DOI 10.1007/s10753-017-0634-1
PII 10.1007/s10753-017-0634-1
PMID 28766178
MeSH Adenosine Triphosphate / pharmacology Animals Cell Line Drug Synergism Inflammasomes / metabolism Interleukin-1beta / analysis Interleukin-1beta / metabolism* Interleukin-6 / analysis Interleukin-6 / metabolism* Lipopolysaccharides / pharmacology Macrophages / metabolism* Mice
IF 3.212
リソース情報
ヒト・動物細胞 RAW 264(RCB0535) J774.1(RCB0434)