Reference - Detail
| RRC ID | 65751 |
|---|---|
| Author | Kokado M, Okada Y, Miyamoto T, Yamanaka O, Saika S. |
| Title | Effects of epiplakin-knockdown in cultured corneal epithelial cells. |
| Journal | BMC Res Notes |
| Abstract |
BACKGROUND:To investigate effects of knockdown of epiplakin gene expression on the homeostasis of cultured corneal epithelial cell line. We previously reported acceleration of corneal epithelial wound healing in an epiplakin-null mouse. METHODS:Gene expression of epiplakin was knockdowned by employing siRNA transfection in SV40-immortalized human corneal epithelial cell line. Protein expression of E-cadherin, keratin 6 and vimentin was examined by western blotting. Cell migration and proliferation were examined by using scratch assay and Alamar blue assay, respectively. RESULTS:Scratch assay and Alamar blue assay showed migration and proliferation of the cells was accelerated by epiplakin knockdown. siRNA-knockdown of epiplakin suppressed protein expression of E-cadherin, keratin 6 and vimentin. CONCLUSIONS:Decreased expression of E-cadherin, keratin 6 and vimentin might be included in the mechanisms of cell migration acceleration in the absence of epiplakin. The mechanism of cell proliferation stimulation by epiplakin knockdown is to be investigated. |
| Volume | 9 |
| Pages | 278 |
| Published | 2016-5-20 |
| DOI | 10.1186/s13104-016-2082-7 |
| PII | 10.1186/s13104-016-2082-7 |
| PMID | 27206504 |
| PMC | PMC4873999 |
| MeSH | Autoantigens / genetics Autoantigens / physiology* Cadherins / metabolism Cell Line, Transformed Epithelium, Corneal / metabolism* Gene Expression Gene Knockdown Techniques Humans Keratin-6 / metabolism Vimentin / metabolism |
| Resource | |
| Human and Animal Cells | HCE-T(RCB2280) |