RRC ID 65761
Author Kusama K, Okamoto Y, Saito K, Kasahara T, Murata T, Ueno Y, Kobayashi Y, Kamada Y, Miyoshi E.
Title Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system.
Journal Glycoconj J
Abstract An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a Pholiota squarrosa lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system. In the current study, we developed an improved PhoSL-ELISA system with higher sensitivity to detect core-fucosylated haptoglobin using high-concentration urea as a denaturing agent with lectin to facilitate detection. We then reevaluated the performance of PhoSL reactive-core-fucosylated haptoglobin (PhoSL-HP) as a PC biomarker using the improved PhoSL-ELISA system. PhoSL-HP levels in the sera of patients with PC were significantly higher than those in healthy volunteers, with an area under the curve (AUC) value of 0.753. Furthermore, the AUC value of CA19-9 improved from 0.793 to 0.907 when combined with PhoSL-HP. Additionally, several CA19-9-negative cases among the patients with PC were diagnosed as positive for PhoSL-HP. In conclusion, PhoSL-HP detection using our improved ELISA system might allow PhoSL-HP to serve as a potential biomarker for PC and thus might be useful to complement the detection of CA19-9 in PC diagnosis.
Volume 34(4)
Pages 537-544
Published 2017-8-1
DOI 10.1007/s10719-017-9772-9
PII 10.1007/s10719-017-9772-9
PMID 28455724
PMC PMC5502058
MeSH Adult Aged Antigens, Tumor-Associated, Carbohydrate / metabolism Biomarkers, Tumor / blood Biomarkers, Tumor / metabolism* Biotinylation Enzyme-Linked Immunosorbent Assay / methods* Female Haptoglobins / metabolism* Hep G2 Cells Humans Lectins / metabolism* Male Middle Aged Pancreatic Neoplasms / blood Pancreatic Neoplasms / metabolism* Pholiota / metabolism* Protein Denaturation
IF 2.197
Human and Animal Cells Hep G2(RCB1648)