論文 - 詳細
| RRC ID | 65779 |
|---|---|
| 著者 | Matsunami A, Mizuno-Kamiya M, Kawaki H, Takayama E, Ueno K, Ando M, Morimoto-Ito H, Muramatsu Y, Sumitomo S, Kondoh N. |
| タイトル | Augmented secretion of IL-1α from mouse oral squamous cell carcinoma (OSCC) vcells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells. |
| ジャーナル | J Oral Biosci |
| Abstract |
OBJECTIVES:We have previously reported that mouse oral squamous carcinoma (OSCC), Sq-1979-1, produces interleukin (IL)-1α, which specifically enhances the immunosuppressive activity of co-cultured mesenchymal stromal 10T1/2 cells. This study assessed the conditions promoting the production of IL-1α in Sq-1979-1 cells, which could further enhance the immunosuppressive function of 10T1/2 cells, and evaluated its expression in OSCC tissues. METHODS:The expression of IL-1α was examined by RT-PCR, western blotting, and enzyme-linked immune sorbent assay (ELISA). The interferon (IFN)- γ-producing capability of anti-CD3 antibody-stimulated mouse spleen cells co-cultured with 10T1/2 cells and conditioned medium (CM) from Sq-1979-1 cells was examined by ELISA. The function of IL-1α was examined using an anti-IL1α antibody. Immunohistochemical analysis of the OSCC tissues was performed. RESULTS:The production of IL-1α from Sq-1979-1 cells was synergistically enhanced in lower serum (0.5% or 1.0% FBS) at the transcriptional level, and under hypoxia (1.0% oxygen) at the release level compared to that in the control medium supplemented with 10% FBS under normoxia. The IFN-γ-producing capability of stimulated spleen cells co-cultured with 10T1/2 cells was significantly reduced in the CMs prepared with the lower serum or under hypoxia. These functions of CMs were completely abolished by the anti-IL-1α antibody. The expression of IL-1α in OSCC tissues was prominent in the midst of a carcinomatous cellular lesion or a nearby necrotic lesion, where a supply deficiency could occur. CONCLUSIONS:IL-1α production by Sq-1979-1 cells was synergistically augmented under low serum and hypoxic conditions, which could promote the immunosuppressive activity of mesenchymal cells. |
| 巻・号 | 63(3) |
| ページ | 284-291 |
| 公開日 | 2021-9-1 |
| DOI | 10.1016/j.job.2021.06.001 |
| PII | S1349-0079(21)00088-8 |
| PMID | 34153475 |
| MeSH | Animals Carcinoma, Squamous Cell* Head and Neck Neoplasms* Hypoxia Mesenchymal Stem Cells* Mice Mouth Neoplasms* Squamous Cell Carcinoma of Head and Neck |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |