| RRC ID |
6581
|
| 著者 |
Kadouchi I, Sakamoto K, Tangjiao L, Murakami T, Kobayashi E, Hoshino Y, Yamaguchi A.
|
| タイトル |
Latexin is involved in bone morphogenetic protein-2-induced chondrocyte differentiation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Latexin is the only known carboxypeptidase A inhibitor in mammals. We previously demonstrated that BMP-2 significantly induced latexin expression in Runx2-deficient mesenchymal cells (RD-C6 cells), during chondrocyte and osteoblast differentiation. In this study, we investigated latexin expression in the skeleton and its role in chondrocyte differentiation. Immunohistochemical studies revealed that proliferating and prehypertrophic chondrocytes expressed latexin during skeletogenesis and bone fracture repair. In the early phase of bone fracture, latexin mRNA expression was dramatically upregulated. BMP-2 upregulated the expression of the mRNAs of latexin, Col2a1, and the gene encoding aggrecan (Agc1) in a micromass culture of C3H10T1/2 cells. Overexpression of latexin additively stimulated the BMP-2-induced expression of the mRNAs of Col2a, Agc1, and Col10a1. BMP-2 treatment upregulated Sox9 expression, and Sox9 stimulated the promoter activity of latexin. These results indicate that latexin is involved in BMP-2-induced chondrocyte differentiation and plays an important role in skeletogenesis and skeletal regeneration.
|
| 巻・号 |
378(3)
|
| ページ |
600-4
|
| 公開日 |
2009-1-16
|
| DOI |
10.1016/j.bbrc.2008.11.111
|
| PII |
S0006-291X(08)02293-6
|
| PMID |
19059214
|
| MeSH |
Aggrecans / genetics
Aggrecans / metabolism
Animals
Antigens / genetics
Antigens / metabolism*
Bone Morphogenetic Protein 2 / pharmacology
Bone Regeneration* / genetics
Cell Differentiation* / genetics
Cell Line
Chondrocytes / cytology*
Chondrocytes / drug effects
Chondrocytes / metabolism
Collagen Type II / genetics
Collagen Type II / metabolism
Collagen Type X / genetics
Collagen Type X / metabolism
Embryo, Mammalian / metabolism
Gene Expression Regulation, Developmental
Mice
Mice, Inbred Strains
Osteogenesis* / genetics
Promoter Regions, Genetic
SOX9 Transcription Factor / genetics
SOX9 Transcription Factor / metabolism
Skeleton
|
| IF |
2.985
|
| 引用数 |
9
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
