| RRC ID |
65835
|
| 著者 |
Izuta S, Yamaguchi S, Misawa R, Yamahira S, Tan M, Kawahara M, Suzuki T, Takagi T, Sato K, Nakamura M, Nagamune T, Okamoto A.
|
| タイトル |
Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Molecular networks on the cytoplasmic faces of cellular plasma membranes are critical research topics in biological sciences and medicinal chemistry. However, the selective permeability of the cell membrane restricts the researchers from accessing to the intact intracellular factors on the membrane from the outside. Here, a microfluidic method to prepare cell membrane sheets was developed as a promising tool for direct examination of the cytoplasmic faces of cell membranes. Mammalian cells immobilized on a poly(ethylene glycol)-lipid coated substrate were rapidly and efficiently fractured, with the sheer stress of laminar flow in microchannels, resulting in isolation of the bottom cell membrane sheets with exposed intact cytoplasmic faces. On these faces of the cell membrane sheets, both ligand-induced phosphorylation of receptor tyrosine kinases and selective enzymatic modification of a G-protein coupling receptor were directly observed. Thus, the present cell membrane sheet should serve as a unique platform for studies providing new insights into juxta-membrane molecular networks and drug discovery.
|
| 巻・号 |
7(1)
|
| ページ |
14962
|
| 公開日 |
2017-11-2
|
| DOI |
10.1038/s41598-017-14737-7
|
| PII |
10.1038/s41598-017-14737-7
|
| PMID |
29097751
|
| PMC |
PMC5668413
|
| MeSH |
Animals
Biocatalysis
Cell Line
Cell Membrane / metabolism*
Cell Membrane / ultrastructure
Cytoplasm / metabolism*
Cytoplasm / ultrastructure
Equipment Design
Humans
Lab-On-A-Chip Devices*
Mice
Micromanipulation / instrumentation*
Microscopy, Confocal
Microscopy, Electron, Scanning
Phosphorylation
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805)
HeLa |
