Reference - Detail
| RRC ID | 6589 |
|---|---|
| Author | Monira P, Koyama Y, Fukutomi R, Yasui K, Isemura M, Yokogoshi H. |
| Title | Effects of Japanese mistletoe lectin on cytokine gene expression in human colonic carcinoma cells and in the mouse intestine. |
| Journal | Biomed Res |
| Abstract |
Mistletoe lectins have various biological activities including anti-cancer and immunomodulatory effects. We previously isolated a lectin (ML-J) from Japanese mistletoe. In the present study, we examined the effects of ML-J on cytokine gene expression in human colon adenocarcinoma Caco-2 cells and in the mouse intestine. The results of reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction indicated that ML-J caused an upregulation of the gene expression of the proinflammatory cytokines interleukin (IL)-8, tumor necrosis factor-alpha (TNF-alpha) and IL-6 in Caco-2 cells and TNF-alpha and IL-6 in the duodenum. This study provides the first example to show that a perorally administered plant lectin affects gene expression in the duodenum. |
| Volume | 30(5) |
| Pages | 303-9 |
| Published | 2009-10-1 |
| DOI | 10.2220/biomedres.30.303 |
| PII | JST.JSTAGE/biomedres/30.303 |
| PMID | 19887727 |
| MeSH | Animals Caco-2 Cells / drug effects* Caco-2 Cells / physiology Cytokines* / genetics Cytokines* / metabolism Gene Expression Regulation / drug effects* Humans Intestines / anatomy & histology Intestines / drug effects* Intestines / physiology Mice Mistletoe / chemistry* Plant Lectins / pharmacology* |
| IF | 1.429 |
| Times Cited | 7 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL |
| Resource | |
| Human and Animal Cells | CACO-2(RCB0988) |