論文 - 詳細
| RRC ID | 65905 |
|---|---|
| 著者 | Takahashi H, Ohkuchi A, Kuwata T, Usui R, Baba Y, Suzuki H, Chaw Kyi TT, Matsubara S, Saito S, Takizawa T. |
| タイトル | Endogenous and exogenous miR-520c-3p modulates CD44-mediated extravillous trophoblast invasion. |
| ジャーナル | Placenta |
| Abstract |
INTRODUCTION:Adequate extravillous trophoblast (EVT) invasion is essential for successful placentation. Although miR-520c-3p plays an important role in CD44-mediated invasion in cancer cells, there is little information on whether miR-520c-3p is involved in the regulatory mechanisms of CD44-mediated EVT invasion. METHODS:We screened first trimester trophoblast cells and trophoblast cell lines for expression of miR-520c-3p using real-time polymerase chain reaction. The cell invasion assay was performed using EVT cell lines, HTR8/SVneo and HChEpC1b, to investigate the capability of suppressing EVT invasion by miR-520c-3p. Laser microdissection analysis was then used to determine whether miR-520c-3p was present in the first trimester decidua. Finally, the possibility of chorionic villous trophoblast (CVT)-EVT communication via exosomal miR-520c-3p was determined using an in vitro model based on BeWo exosomes and the EVT cell lines as recipient cells. RESULTS:The miR-520c-3p level was significantly downregulated in EVT cell lines and EVTs. Cell invasion was significantly inhibited in miR-520c-3p-overexpressing cell lines, involving a significant reduction of CD44. Laser microdissection analysis showed that miR-520c-3p in the periarterial area of the decidua was significantly higher than that in the non-periarterial area. Using an in vitro model system, BeWo exosomal miR-520c-3p was internalized into the EVT cells with subsequently reduced cell invasion via CD44 repression. CONCLUSIONS:EVT invasion is synergistically enhanced by the reciprocal expression of endogenous miR-520c-3p and CD44. The present study supports a novel model involving a placenta-associated miRNA function in cell-cell communication in which CVT exosomal miR-520c-3p regulates cell invasion by targeting CD44 in EVTs. |
| 巻・号 | 50 |
| ページ | 25-31 |
| 公開日 | 2017-2-1 |
| DOI | 10.1016/j.placenta.2016.12.016 |
| PII | S0143-4004(16)30663-4 |
| PMID | 28161058 |
| MeSH | Cell Line Cell Movement / physiology* Female Humans Hyaluronan Receptors / metabolism* MicroRNAs / genetics MicroRNAs / metabolism* Placenta / metabolism Pregnancy Pregnancy Trimester, First Trophoblasts / cytology Trophoblasts / metabolism* |
| IF | 3.177 |
| リソース情報 | |
| ヒト・動物細胞 | BeWo(RCB1644) |