論文 - 詳細
| RRC ID | 65922 |
|---|---|
| 著者 | Shubhra QTH, Oyane A, Araki H, Nakamura M, Tsurushima H. |
| タイトル | Calcium phosphate nanoparticles prepared from infusion fluids for stem cell transfection: process optimization and cytotoxicity analysis. |
| ジャーナル | Biomater Sci |
| Abstract |
This is the first study to report the use of infusion fluids for particle-mediated gene delivery with DNA-immobilized calcium phosphate (CaP) nanoparticles (NPs). In conventional CaP systems, CaP NPs are fabricated in labile supersaturated CaP solutions which are prepared from chemical reagents. In the present study, we fabricated CaP NPs via coprecipitation in labile supersaturated CaP solutions that were prepared from infusion fluids (even the water used was of injectable quality) instead of chemical reagents and demonstrated their gene delivery capabilities for the hard to transfect pluripotent stem cell (C3H10T1/2) along with the easy to transfect CHO-K1 cell. To achieve a high gene delivery capability by keeping the high safety level of our system intact, we varied the process parameters: coprecipitation temperature and time, along with the Ca and P concentrations of the CaP solution, without using additive agents (e.g. surfactants) other than infusion fluids and plasmids. The optimization of these process parameters led to a higher gene delivery capability compared with that of a commercial CaP system for both types of cells. MTT and protein assays showed that both our system and the commercial CaP system were not cytotoxic to both types of cells. Our CaP system has the advantages of high biological safety (due to injectable source materials), high serum-resistance, and relatively high and controllable gene delivery capability, depending on the process parameters. Thus, the present system warrants consideration for gene delivery applications. |
| 巻・号 | 5(5) |
| ページ | 972-981 |
| 公開日 | 2017-5-2 |
| DOI | 10.1039/c6bm00870d |
| PMID | 28211929 |
| MeSH | Animals CHO Cells Calcium Phosphates / chemistry* Cell Line Cell Survival Chemical Precipitation Cricetulus DNA / administration & dosage* DNA / genetics Mice Nanoparticles / chemistry* Nanoparticles / ultrastructure Plasmids / administration & dosage* Plasmids / genetics Stem Cells / metabolism* Temperature Transfection / methods* |
| IF | 6.183 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) 10T1/2(RCB0247) |