| RRC ID |
65981
|
| 著者 |
Nakamura M, Yoshida H, Moriyama Y, Kawakita I, Wlizla M, Takebayashi-Suzuki K, Horb ME, Suzuki A.
|
| タイトル |
TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Amphibians such as Xenopus tropicalis exhibit a remarkable capacity for tissue regeneration after traumatic injury. Although transforming growth factor-β (TGF-β) receptor signaling is known to be essential for tissue regeneration in fish and amphibians, the role of TGF-β ligands in this process is not well understood. Here, we show that inhibition of TGF-β1 function prevents tail regeneration in Xenopus tropicalis tadpoles. We found that expression of tgfb1 is present before tail amputation and is sustained throughout the regeneration process. CRISPR-mediated knock-out (KO) of tgfb1 retards tail regeneration; the phenotype of tgfb1 KO tadpoles can be rescued by injection of tgfb1 mRNA. Cell proliferation, a critical event for the success of tissue regeneration, is downregulated in tgfb1 KO tadpoles. In addition, tgfb1 KO reduces the expression of phosphorylated Smad2/3 (pSmad2/3) which is important for TGF-β signal-mediated cell proliferation. Collectively, our results show that TGF-β1 regulates cell proliferation through the activation of Smad2/3. We therefore propose that TGF-β1 plays a critical role in TGF-β receptor-dependent tadpole tail regeneration in Xenopus.
|
| 巻・号 |
565
|
| ページ |
91-96
|
| 公開日 |
2021-8-6
|
| DOI |
10.1016/j.bbrc.2021.05.082
|
| PII |
S0006-291X(21)00873-1
|
| PMID |
34102475
|
| PMC |
PMC8255271
|
| MeSH |
Animals
Cell Proliferation
Larva / metabolism*
Signal Transduction
Smad2 Protein / metabolism
Smad3 Protein / metabolism
Transforming Growth Factor beta1 / metabolism*
Xenopus
Xenopus Proteins / metabolism
|
| IF |
2.985
|
| リソース情報 |
| ツメガエル・イモリ |
|
