論文 - 詳細
| RRC ID | 65981 |
|---|---|
| 著者 | Nakamura M, Yoshida H, Moriyama Y, Kawakita I, Wlizla M, Takebayashi-Suzuki K, Horb ME, Suzuki A. |
| タイトル | TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Amphibians such as Xenopus tropicalis exhibit a remarkable capacity for tissue regeneration after traumatic injury. Although transforming growth factor-β (TGF-β) receptor signaling is known to be essential for tissue regeneration in fish and amphibians, the role of TGF-β ligands in this process is not well understood. Here, we show that inhibition of TGF-β1 function prevents tail regeneration in Xenopus tropicalis tadpoles. We found that expression of tgfb1 is present before tail amputation and is sustained throughout the regeneration process. CRISPR-mediated knock-out (KO) of tgfb1 retards tail regeneration; the phenotype of tgfb1 KO tadpoles can be rescued by injection of tgfb1 mRNA. Cell proliferation, a critical event for the success of tissue regeneration, is downregulated in tgfb1 KO tadpoles. In addition, tgfb1 KO reduces the expression of phosphorylated Smad2/3 (pSmad2/3) which is important for TGF-β signal-mediated cell proliferation. Collectively, our results show that TGF-β1 regulates cell proliferation through the activation of Smad2/3. We therefore propose that TGF-β1 plays a critical role in TGF-β receptor-dependent tadpole tail regeneration in Xenopus. |
| 巻・号 | 565 |
| ページ | 91-96 |
| 公開日 | 2021-8-6 |
| DOI | 10.1016/j.bbrc.2021.05.082 |
| PII | S0006-291X(21)00873-1 |
| PMID | 34102475 |
| PMC | PMC8255271 |
| MeSH | Animals Cell Proliferation Larva / metabolism* Signal Transduction Smad2 Protein / metabolism Smad3 Protein / metabolism Transforming Growth Factor beta1 / metabolism* Xenopus Xenopus Proteins / metabolism |
| IF | 2.985 |
| オルトメトリクス指標 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ツメガエル・イモリ | |