論文 - 詳細
| RRC ID | 65997 |
|---|---|
| 著者 | Račić A, Čalija B, Milić J, Jurišić Dukovski B, Lovrić J, Dobričić V, Micov A, Vuković M, Stepanović-Petrović R, Krajišnik D. |
| タイトル | Formulation of olopatadine hydrochloride viscous eye drops - physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches. |
| ジャーナル | Eur J Pharm Sci |
| Abstract |
The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their combination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic preparations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine. |
| 巻・号 | 166 |
| ページ | 105906 |
| 公開日 | 2021-11-1 |
| DOI | 10.1016/j.ejps.2021.105906 |
| PII | S0928-0987(21)00207-4 |
| PMID | 34118409 |
| MeSH | Animals Biological Products* Mice Olopatadine Hydrochloride Ophthalmic Solutions Spectroscopy, Fourier Transform Infrared Viscosity |
| IF | 3.616 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |