Author Takahashi M, Hakamata Y, Murakami T, Takeda S, Kaneko T, Takeuchi K, Takahashi R, Ueda M, Kobayashi E.
Title Establishment of lacZ-transgenic rats: a tool for regenerative research in myocardium.
Journal Biochem. Biophys. Res. Commun.
Abstract Animals transgenic (Tg) for reporter genes would be useful to following a given cell lineage during differentiation and regeneration processes. Here, we established a beta-galactosidase (lacZ) Tg rat to use as a tool for regenerative research. Strong lacZ expression was observed in the skeletal muscles, myocardium, pancreas, and skin obtained from these lacZ-Tg rats, and moderate lacZ expression was observed in the liver, spleen, kidney, and cartilage. In contrast, brain, vessels, lung, adrenal gland, small intestine, blood leukocytes, bone marrow (BM) cells, and peripheral blood cells showed no lacZ expression. To test whether this lacZ-Tg rat could be used for regenerative research in myocardium, we induced myocardial injury after a lacZ-Tg BM transplant (BMT) into wild-type rats. The results show that lacZ-positive cardiomyocytes were found in the peri-infarct and uninjured myocardium in the BMT recipient rats. These findings suggest that lacZ-Tg rats are useful tool for regenerative research in the myocardium.
Volume 305(4)
Pages 904-8
Published 2003-6-13
PII S0006291X03008416
PMID 12767916
MeSH Animals Animals, Genetically Modified Animals, Newborn Bone Marrow Cells / physiology Bone Marrow Transplantation Cardiomyopathies / etiology Cell Differentiation Genes, Reporter* Male Models, Animal* Myocytes, Cardiac / cytology Myocytes, Cardiac / physiology* Rats / anatomy & histology Rats / genetics* Rats / metabolism Rats, Wistar Regeneration* Tissue Distribution beta-Galactosidase / analysis beta-Galactosidase / genetics*
IF 2.705
Times Cited 28
Rats DA-Tg(CAG-lacZ)19Jmsk(strainID=530) DA-Tg(CAG-lacZ)30Jmsk(strainID=531)