論文 - 詳細
| RRC ID | 66033 |
|---|---|
| 著者 | Waku T, Imanishi Y, Yoshino Y, Kunugi S, Serizawa T, Tanaka N. |
| タイトル | Fusion of polymeric material-binding peptide to cell-adhesion artificial proteins enhances their biological function. |
| ジャーナル | Biointerphases |
| Abstract |
Orientation-controlled protein immobilization on a solid substrate surface is important for the development of biomedical materials such as scaffolds used in tissue engineering. In this study, the authors demonstrated that the introduction of material-binding peptides (MBPs) in Arg-Gly-Asp (RGD)-fused artificial proteins called blocking peptide fragment (BPF), which are fragments (residues 419-607) of the molecular chaperone DnaK, enhances the oriented adsorption of proteins on the polymer surface and improves their cell adhesion capability. The authors used isotactic poly(methyl methacrylate) (it-PMMA) binding peptides (c02 peptide; ELWRPTR) as a model system. A quartz crystal microbalance study showed that the fusion of c02 peptide with BPF-RGD proteins slightly enhanced adsorption on it-PMMA surfaces. On the other hand, atomic force microscopic images of it-PMMA surfaces adsorbed with c02-BPF-RGD proteins showed a dotlike pattern, with the sizes of the dots comparable to those of BPF protein dimers, indicating that the immobilization of c02-BPF-RGD partially occurred in an oriented manner via specific interaction between the c02 peptide and it-PMMA. This is in sharp contrast to the random adsorption of BPF-RGD and BPF. These results were supported by results of the enzyme-linked immunosorbent assay using an antihistidine tag antibody. In addition, c02-BPF-RGD adsorbed on it-PMMA showed better cell attachment and spreading ability than BPF-RGD and BPF. This methodology can be applied to other MBP systems and cell-binding motifs. Thus, BPF-based artificial cell adhesion proteins fused with MBPs might be useful as surface modifiers of polymer materials for improving their cell adhesion ability. |
| 巻・号 | 12(2) |
| ページ | 021002 |
| 公開日 | 2017-4-24 |
| DOI | 10.1116/1.4979577 |
| PMID | 28438025 |
| MeSH | Adsorption Amino Acid Sequence Animals Biocompatible Materials / chemistry* Biocompatible Materials / pharmacology Cell Adhesion / drug effects Circular Dichroism Mice Microscopy, Atomic Force Molecular Chaperones / chemistry NIH 3T3 Cells Oligopeptides / chemistry Peptides / chemistry* Polymethyl Methacrylate / chemistry* Quartz Crystal Microbalance Techniques Surface Properties |
| IF | 2.043 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3 |