論文 - 詳細
| RRC ID | 66038 |
|---|---|
| 著者 | Ikeda Y, Kawai K, Ikawa A, Kawamoto K, Egami Y, Araki N. |
| タイトル | Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. |
| ジャーナル | J Cell Sci |
| Abstract |
Lamellipodia are sheet-like cell protrusions driven by actin polymerization mainly through Rac1, a GTPase molecular switch. In Fcγ receptor-mediated phagocytosis of IgG-opsonized erythrocytes (IgG-Es), Rac1 activation is required for lamellipodial extension along the surface of IgG-Es. However, the significance of Rac1 deactivation in phagosome formation is poorly understood. Our live-cell imaging and electron microscopy revealed that RAW264 macrophages expressing a constitutively active Rac1 mutant showed defects in phagocytic cup formation, while lamellipodia were formed around IgG-Es. Because activated Rac1 reduced the phosphorylation levels of myosin light chains, failure of the cup formation is probably due to inhibition of actin/myosin II contractility. Reversible photo-manipulation of the Rac1 switch in macrophages fed with IgG-Es could phenocopy two lamellipodial motilities: outward-extension and cup-constriction by Rac1 ON and OFF, respectively. In conjunction with fluorescence resonance energy transfer imaging of Rac1 activity, we provide a novel mechanistic model of phagosome formation spatiotemporally controlled by Rac1 switching within a phagocytic cup. |
| 巻・号 | 130(15) |
| ページ | 2530-2540 |
| 公開日 | 2017-8-1 |
| DOI | 10.1242/jcs.201749 |
| PII | jcs.201749 |
| PMID | 28600322 |
| MeSH | Animals Macrophages / immunology* Mice Models, Immunological* Neuropeptides / immunology* Phagosomes / immunology* Phosphorylation / immunology RAW 264.7 Cells Receptors, IgG / immunology* rac1 GTP-Binding Protein / immunology* |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |