| RRC ID |
66047
|
| 著者 |
Miki K, Enomoto A, Inoue T, Nabeshima T, Saino S, Shimizu S, Matsuoka H, Ohe K.
|
| タイトル |
Polymeric Self-Assemblies with Boron-Containing Near-Infrared Dye Dimers for Photoacoustic Imaging Probes.
|
| ジャーナル |
Biomacromolecules
|
| Abstract |
Polymers containing pyrrolopyrrole aza-BODIPY (PPAB) and thiophene-bridged BODIPY dimers (TBD) having poly(ethylene glycol) (PEG) or PEGylated hyaluronic acid (HA) were prepared by facile conjugation approaches. Self-assemblies consisting of TBD-conjugated polymers more efficiently generated photoacoustic (PA) signals than PPAB-PEG conjugate upon irradiation with near-infrared pulsed laser light. Among dye-conjugated polymers examined, TBD-HA-PEG conjugates efficiently generated photoacoustic signals, 1.49-1.83 times stronger than that of commercially available indocyanine green (ICG). We found that the following two factors are essential to enhance PA signals from self-assemblies: (1) the formation of strongly interacting TBD aggregates and (2) enhancement of the elastic modulus of self-assemblies by conjugating TBDs with HA. TBD-conjugated HA derivatives circulated in blood vessels for a longer time (15.6 ± 4.9% injected dose (ID) in blood 24 h after injection) and more specifically accumulated in tumor tissues (17.8 ± 3.5% ID/g in tumor 24 h after injection) than ICG-conjugated HA derivatives, visualizing a tumor site more clearly. The cell uptake experiment of dye-HA conjugates indicates that ICG-conjugated polymers internalized into cells or merged with cell walls to emit strong fluorescence, while TBD-conjugated polymers were not internalized into cells. Because the disassembly of the TBD-conjugated HA derivatives is suppressed, aggregated TBDs emit weak fluorescence but efficiently generate strong PA signals in tumor tissues.
|
| 巻・号 |
18(1)
|
| ページ |
249-256
|
| 公開日 |
2017-1-9
|
| DOI |
10.1021/acs.biomac.6b01568
|
| PII |
10.1021/acs.biomac.6b01568
|
| PMID |
27936606
|
| MeSH |
Animals
Boron / chemistry*
Colonic Neoplasms / pathology*
Coloring Agents / chemistry*
Hyaluronic Acid / chemistry
Indocyanine Green / chemistry
Mice
Mice, Inbred BALB C
Mice, Nude
Optical Imaging / methods*
Photoacoustic Techniques / methods*
Polymers / chemistry*
Spectroscopy, Near-Infrared
Tumor Cells, Cultured
|
| IF |
6.092
|
| リソース情報 |
| ヒト・動物細胞 |
Colon-26(RCB2657) |
