論文 - 詳細
| RRC ID | 66047 |
|---|---|
| 著者 | Miki K, Enomoto A, Inoue T, Nabeshima T, Saino S, Shimizu S, Matsuoka H, Ohe K. |
| タイトル | Polymeric Self-Assemblies with Boron-Containing Near-Infrared Dye Dimers for Photoacoustic Imaging Probes. |
| ジャーナル | Biomacromolecules |
| Abstract |
Polymers containing pyrrolopyrrole aza-BODIPY (PPAB) and thiophene-bridged BODIPY dimers (TBD) having poly(ethylene glycol) (PEG) or PEGylated hyaluronic acid (HA) were prepared by facile conjugation approaches. Self-assemblies consisting of TBD-conjugated polymers more efficiently generated photoacoustic (PA) signals than PPAB-PEG conjugate upon irradiation with near-infrared pulsed laser light. Among dye-conjugated polymers examined, TBD-HA-PEG conjugates efficiently generated photoacoustic signals, 1.49-1.83 times stronger than that of commercially available indocyanine green (ICG). We found that the following two factors are essential to enhance PA signals from self-assemblies: (1) the formation of strongly interacting TBD aggregates and (2) enhancement of the elastic modulus of self-assemblies by conjugating TBDs with HA. TBD-conjugated HA derivatives circulated in blood vessels for a longer time (15.6 ± 4.9% injected dose (ID) in blood 24 h after injection) and more specifically accumulated in tumor tissues (17.8 ± 3.5% ID/g in tumor 24 h after injection) than ICG-conjugated HA derivatives, visualizing a tumor site more clearly. The cell uptake experiment of dye-HA conjugates indicates that ICG-conjugated polymers internalized into cells or merged with cell walls to emit strong fluorescence, while TBD-conjugated polymers were not internalized into cells. Because the disassembly of the TBD-conjugated HA derivatives is suppressed, aggregated TBDs emit weak fluorescence but efficiently generate strong PA signals in tumor tissues. |
| 巻・号 | 18(1) |
| ページ | 249-256 |
| 公開日 | 2017-1-9 |
| DOI | 10.1021/acs.biomac.6b01568 |
| PII | 10.1021/acs.biomac.6b01568 |
| PMID | 27936606 |
| MeSH | Animals Boron / chemistry* Colonic Neoplasms / pathology* Coloring Agents / chemistry* Hyaluronic Acid / chemistry Indocyanine Green / chemistry Mice Mice, Inbred BALB C Mice, Nude Optical Imaging / methods* Photoacoustic Techniques / methods* Polymers / chemistry* Spectroscopy, Near-Infrared Tumor Cells, Cultured |
| IF | 6.092 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Colon-26(RCB2657) |