論文 - 詳細
| RRC ID | 66068 |
|---|---|
| 著者 | Nyunoya H, Noda T, Kawamoto Y, Hayashi Y, Ishibashi Y, Ito M, Okino N. |
| タイトル | Lack of ∆5 Desaturase Activity Impairs EPA and DHA Synthesis in Fish Cells from Red Sea Bream and Japanese Flounder. |
| ジャーナル | Mar Biotechnol (NY) |
| Abstract |
Long-chain (≥ C20) polyunsaturated fatty acids (LC-PUFA), such as eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), are necessary for human health and are obtained from marine fish-derived oils. Marine fish are LC-PUFA-rich animals; however, many of them require LC-PUFA for growth. Therefore, it is suggested that they do not have sufficient ability to biosynthesize LC-PUFA. To evaluate in vivo LC-PUFA synthetic activity in fish cells, fish-derived cell lines from red sea bream (Pagrus major, PMS and PMF), Japanese flounder (Paralichthys olivaceus, HINAE), and zebrafish (Danio rerio, BRF41) were incubated with n-3 fatty acids labeled by radioisotopes or stable isotopes, and then, n-3 PUFA were analyzed by thin-layer chromatography or liquid chromatography-mass spectrometry. Labeled EPA and DHA were biosynthesized from labeled α-linolenic acid (18:3n-3) in BRF41, whereas they were not detected in PMS, PMF, or HINAE cells. We next cloned the fatty acid desaturase 2 (Fads2) cDNAs from PMF cells and zebrafish, expressed in budding yeasts, and then analyzed the substrate specificities of enzymes. As a result, we found that Fads2 from PMF cells was a ∆6/∆8 desaturase. Collectively, our study indicates that cell lines from red sea bream and Japanese flounder were not able to synthesize EPA or DHA by themselves, possibly due to the lack of ∆5 desaturase activity. Furthermore, this study provides a sensitive and reproducible non-radioactive method for evaluating LC-PUFA synthesis in fish cells using a stable isotope and liquid chromatography-mass spectrometry. |
| 巻・号 | 23(3) |
| ページ | 472-481 |
| 公開日 | 2021-6-1 |
| DOI | 10.1007/s10126-021-10040-9 |
| PII | 10.1007/s10126-021-10040-9 |
| PMID | 34176006 |
| MeSH | Animals Cell Line Delta-5 Fatty Acid Desaturase Fatty Acid Desaturases / deficiency* Fatty Acids, Omega-3 / metabolism Fatty Acids, Unsaturated / biosynthesis* Flounder / metabolism* Sea Bream / metabolism* Zebrafish / metabolism* |
| IF | 2.798 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BRF41(RCB0804) |