RRC ID 66077
Author Lahr CA, Landgraf M, Wagner F, Cipitria A, Moreno-Jiménez I, Bas O, Schmutz B, Meinert C, Cavalcanti ADS, Mashimo T, Miyasaka Y, Holzapfel BM, Shafiee A, McGovern JA, Hutmacher DW.
Title A humanised rat model of osteosarcoma reveals ultrastructural differences between bone and mineralised tumour tissue.
Journal Bone
Abstract Current xenograft animal models fail to accurately replicate the complexity of human bone disease. To gain translatable and clinically valuable data from animal models, new in vivo models need to be developed that mimic pivotal aspects of human bone physiology as well as its diseased state. Above all, an advanced bone disease model should promote the development of new treatment strategies and facilitate the conduction of common clinical interventional procedures. Here we describe the development and characterisation of an orthotopic humanised tissue-engineered osteosarcoma (OS) model in a recently genetically engineered x-linked severe combined immunodeficient (X-SCID) rat. For the first time in a genetically modified rat, our results show the successful implementation of an orthotopic humanised tissue-engineered bone niche supporting the growth of a human OS cell line including its metastatic spread to the lung. Moreover, we studied the inter- and intraspecies differences in ultrastructural composition of bone and calcified tissue produced by the tumour, pointing to the crucial role of humanised animal models.
Volume 158
Pages 116018
Published 2022-5-1
DOI 10.1016/j.bone.2021.116018
PII S8756-3282(21)00180-0
PMID 34023543
MeSH Animals Bone Neoplasms* / secondary Bone and Bones / pathology Cell Line Cell Line, Tumor Humans Osteosarcoma* / drug therapy Rats Tissue Engineering
IF 4.147
Rats F344 rats with X-SCID phenotype