RRC ID 66098
著者 Yamamoto S, Ushio S, Egashira N, Kawashiri T, Mitsuyasu S, Higuchi H, Ozawa N, Masuguchi K, Ono Y, Masuda S.
タイトル Excessive spinal glutamate transmission is involved in oxaliplatin-induced mechanical allodynia: a possibility for riluzole as a prophylactic drug.
ジャーナル Sci Rep
Abstract Oxaliplatin, a chemotherapy medication, causes severe peripheral neuropathy. Although oxaliplatin-induced peripheral neuropathy is a dose-limiting toxicity, a therapeutic strategy against its effects has not been established. We previously reported the involvement of N-methyl-D-aspartate receptors and their intracellular signalling pathway in oxaliplatin-induced mechanical allodynia in rats. The aim of this study was to clarify the involvement of spinal glutamate transmission in oxaliplatin-induced mechanical allodynia. In vivo spinal microdialysis revealed that the baseline glutamate concentration was elevated in oxaliplatin-treated rats, and that mechanical stimulation of the hind paw markedly increased extracellular glutamate concentration in the same rats. In these rats, the expression of glutamate transporter 1 (GLT-1), which plays a major role in glutamate uptake, was decreased in the spinal cord. Moreover, we explored the potential of pharmacological therapy targeting maintenance of extracellular glutamate homeostasis. The administration of riluzole, an approved drug for amyotrophic lateral sclerosis, suppressed the increase of glutamate concentration, the decrease of GLT-1 expression and the development of mechanical allodynia. These results suggest that oxaliplatin disrupts the extracellular glutamate homeostasis in the spinal cord, which may result in neuropathic symptoms, and support the use of riluzole for prophylaxis of oxaliplatin-induced mechanical allodynia.
巻・号 7(1)
ページ 9661
公開日 2017-8-29
DOI 10.1038/s41598-017-08891-1
PII 10.1038/s41598-017-08891-1
PMID 28851920
PMC PMC5574967
MeSH Animals Antineoplastic Agents / adverse effects* Biological Transport Chemoprevention / methods* Disease Models, Animal Excitatory Amino Acid Antagonists / administration & dosage* Glutamates / metabolism* Hyperalgesia / chemically induced* Hyperalgesia / physiopathology Hyperalgesia / prevention & control Oxaliplatin / adverse effects* Rats Riluzole / administration & dosage* Treatment Outcome
IF 3.998
リソース情報
ヒト・動物細胞 Colon-26(RCB2657)