| RRC ID |
66115
|
| Author |
Sakamoto H, Kita K, Matsuzaki M.
|
| Title |
A novel 2A-peptide-containing plasmid to generate stable Perkinsus marinus cells expressing organelle-targeted genes.
|
| Journal |
J Eukaryot Microbiol
|
| Abstract |
Genetic manipulation techniques for marine protists are not well-established, despite immense efforts. However, Perkinsus marinus is an exception and can be developed as a genetically tractable model organism for related protists. Here, we designed a new plasmid for P. marinus that allows two proteins from a single mRNA to be differently localized using a self-cleaving 2A peptide. This enabled us to establish a stable transfectant expressing a mitochondrially targeted fluorescent protein. The system can be applied to any protein in theory and would make a powerful tool for investigating unique organelles in P. marinus and related dinoflagellates.
|
| Volume |
68(5)
|
| Pages |
e12861
|
| Published |
2021-9-1
|
| DOI |
10.1111/jeu.12861
|
| PMID |
34051022
|
| MeSH |
Apicomplexa* / genetics
Dinoflagellida* / genetics
Organelles / genetics
Peptides
Plasmids / genetics
|
| IF |
2.143
|
| Resource |
| DNA material |
pMGB (RDB15789)
pMGP (RDB15790)
pMRB (RDB15791) |
