RRC ID 66115
Author Sakamoto H, Kita K, Matsuzaki M.
Title A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes.
Journal J Eukaryot Microbiol
Abstract Genetic manipulation techniques for marine protists are not well-established, despite immense efforts. However, Perkinsus marinus is an exception and can be developed as a genetically tractable model organism for related protists. Here, we designed a new plasmid for P. marinus that allows two proteins from a single mRNA to be differently localized using a self-cleaving 2A peptide. This enabled us to establish a stable transfectant expressing a mitochondrially targeted fluorescent protein. The system can be applied to any protein in theory and would make a powerful tool for investigating unique organelles in P. marinus and related dinoflagellates.
Pages e12861
Published 2021-5-29
DOI 10.1111/jeu.12861
PMID 34051022
IF 2.143
DNA material pMGB (RDB15789) pMGP (RDB15790) pMRB (RDB15791)