論文 - 詳細
| RRC ID | 66125 |
|---|---|
| 著者 | Iwakawa HO, Lam AYW, Mine A, Fujita T, Kiyokawa K, Yoshikawa M, Takeda A, Iwasaki S, Tomari Y. |
| タイトル | Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants. |
| ジャーナル | Cell Rep |
| Abstract |
The path of ribosomes on mRNAs can be impeded by various obstacles. One such example is halting of ribosome movement by microRNAs, but the exact mechanism and physiological role remain unclear. Here, we find that ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates production of secondary small interfering RNAs (siRNAs) in plants. We show that the double-stranded RNA-binding protein SGS3 interacts directly with the 3' end of the microRNA in an Argonaute protein, resulting in ribosome stalling. Importantly, microRNA-mediated ribosome stalling correlates positively with efficient production of secondary siRNAs from target mRNAs. Our results illustrate a role of paused ribosomes in regulation of small RNA function that may have broad biological implications across the plant kingdom. |
| 巻・号 | 35(13) |
| ページ | 109300 |
| 公開日 | 2021-6-29 |
| DOI | 10.1016/j.celrep.2021.109300 |
| PII | S2211-1247(21)00676-8 |
| PMID | 34192539 |
| MeSH | Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / metabolism* Argonaute Proteins / metabolism* Base Sequence Cell Line Enhancer Elements, Genetic / genetics MicroRNAs / genetics MicroRNAs / metabolism* Models, Biological Protein Binding RNA, Double-Stranded / metabolism RNA, Plant / genetics RNA, Plant / metabolism* RNA, Small Interfering / metabolism* RNA-Induced Silencing Complex / metabolism Ribosomes / metabolism* |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |