RRC ID 66126
Author Ma Z, Liu X, Nath S, Sun H, Tran TM, Yang L, Mayor S, Miao Y.
Title Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling.
Journal Cell Rep
Abstract Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv. campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI). Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale. By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling. Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
Volume 34(13)
Pages 108884
Published 2021-3-30
DOI 10.1016/j.celrep.2021.108884
PII S2211-1247(21)00198-4
PMID 33789103
MeSH Actins / metabolism* Arabidopsis / microbiology Bacterial Proteins / metabolism* Cell Wall / metabolism Cellulose / metabolism Flagellin / metabolism* Formins / metabolism* Host-Pathogen Interactions Models, Biological Nanoparticles / chemistry* Pathogen-Associated Molecular Pattern Molecules / metabolism Protein Binding Signal Transduction* Xanthomonas campestris / metabolism
IF 8.109
Arabidopsis / Cultured plant cells, genes rpc00008