| RRC ID |
66138
|
| 著者 |
Oda S, Mikami S, Urushihara Y, Murata Y, Kamei Y, Deguchi T, Kitano T, Fujimori KE, Yuba S, Todo T, Mitani H.
|
| タイトル |
Identification of a functional medaka heat shock promoter and characterization of its ability to induce exogenous gene expression in medaka in vitro and in vivo.
|
| ジャーナル |
Zoolog Sci
|
| Abstract |
Heat shock protein promoters (hsp promoters) are powerful tools for investigating gene functions, as the expression of targeted genes can be controlled simply by heating. However, there have been no reports of the utilization of an endogeneous medaka (Oryzias latipes) hsp promoter to induce exogenous gene expression in medaka. We identified and cloned a functional medaka hsp promoter (olphsp70.1) and verified its ability to act as an inducible promoter both in vitro and in vivo. The hsp promoter efficiently induced exogenous gene expression in cultured cells, developing embryos, and also in adult fishes. When used to control the expression of Venus, a variant of yellow fluorescent protein, in transgenic medaka, the hsp promoter was functional in all tissues except for the gonads of adults. These results indicate that the medaka hsp promoter can be a powerful tool for inducing exogenous gene expression and investigating gene functions both in vitro and in vivo in medaka.
|
| 巻・号 |
27(5)
|
| ページ |
410-5
|
| 公開日 |
2010-5-1
|
| DOI |
10.2108/zsj.27.410
|
| PMID |
20443688
|
| MeSH |
Animals
Animals, Genetically Modified
Cell Line
Cloning, Molecular
Embryo, Nonmammalian
Gene Expression Regulation / physiology*
Heat-Shock Proteins / genetics
Heat-Shock Proteins / metabolism*
Luciferases / genetics
Luciferases / metabolism
Oryzias / genetics*
Oryzias / metabolism*
Promoter Regions, Genetic*
|
| IF |
0.843
|
| リソース情報 |
| メダカ |
T5-Tg(olphsp70.1-hRluc-Venus) (TG908)
olvas-DsRedExpress (TG1403)
T5 (MT827)
Cab |
