| RRC ID |
66161
|
| Author |
Sato M, Inohaya A, Yasuda E, Mogami H, Chigusa Y, Kawasaki K, Kawamura Y, Ueda Y, Takai H, Mandai M, Kondoh E.
|
| Title |
Three-dimensional human placenta-like bud synthesized from induced pluripotent stem cells.
|
| Journal |
Sci Rep
|
| Abstract |
Placental dysfunction is related to the pathogenesis of preeclampsia and fetal growth restriction, but there is no effective treatment for it. Recently, various functional three-dimensional organs have been generated from human induced-pluripotent cells (iPSCs), and the transplantation of these iPSCs-derived organs has alleviated liver failure or diabetes mellitus in mouse models. Here we successfully generated a three-dimensional placental organ bud from human iPSCs. The iPSCs differentiated into various lineages of trophoblasts such as cytotrophoblast-like, syncytiotrophoblast-like, and extravillous trophoblast-like cells, forming organized layers in the bud. Placental buds were transplanted to the murine uterus, where 22% of the buds were successfully engrafted. These iPSC-derived placental organ buds could serve as a new model for the study of placental function and pathology.
|
| Volume |
11(1)
|
| Pages |
14167
|
| Published |
2021-7-8
|
| DOI |
10.1038/s41598-021-93766-9
|
| PII |
10.1038/s41598-021-93766-9
|
| PMID |
34239021
|
| PMC |
PMC8266876
|
| MeSH |
Animals
Biomarkers / metabolism
Bone Morphogenetic Protein 4 / pharmacology
Cell Differentiation / drug effects
Cell Line
Cell Lineage / drug effects
Female
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
Mice, Inbred NOD
Mice, SCID
Placenta / cytology*
Placenta / drug effects
Placenta / transplantation
Pregnancy
Trophoblasts / cytology
Trophoblasts / drug effects
Trophoblasts / metabolism
Uterus / physiology
|
| IF |
3.998
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063)
409B2(HPS0076) |
