| RRC ID |
66163
|
| Author |
Yanagi K, Komatsu T, Ogihara S, Okabe T, Kojima H, Nagano T, Ueno T, Hanaoka K, Urano Y.
|
| Title |
Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells.
|
| Journal |
Cell Rep
|
| Abstract |
In this study, we present a live-cell-based fluorometric coupled assay system to identify the compounds that can regulate the targeted metabolic pathways in live cells. The assay is established through targeting specific metabolic pathways and using "input" and "output" metabolite pairs. The changes in the extracellular output that are generated and released into the extracellular media from the input are assessed as the activity of the pathway. The screening for the glycolytic pathway and amino acid metabolism reveals the activities of the present drugs, 6-BIO and regorafenib, that regulate the metabolic fate of tumor cells.
|
| Volume |
36(1)
|
| Pages |
109311
|
| Published |
2021-7-6
|
| DOI |
10.1016/j.celrep.2021.109311
|
| PII |
S2211-1247(21)00687-2
|
| PMID |
34233188
|
| MeSH |
Amino Acids / metabolism
Biological Assay / methods*
Cell Line, Tumor
Cell Survival / drug effects
Cells / metabolism*
Fluorescent Dyes / chemistry
Fluorescent Dyes / metabolism
Glycolysis / drug effects
Humans
Metabolome / drug effects
Phenylurea Compounds / pharmacology
Pyridines / pharmacology
Sorafenib / pharmacology
|
| IF |
8.109
|
| Resource |
| Human and Animal Cells |
MIA Paca2(RCB2094)
HL-60(RCB3683) |
