RRC ID 66196
著者 Aiba K, Kobayashi Y, Aoki S.
タイトル Bioluminescence burst caused by a process in carbohydrate metabolism in a luciferase reporter strain of Escherichia coli.
ジャーナル Biochem Biophys Res Commun
Abstract We previously reported that Escherichia coli strains carrying a firefly luciferase reporter gene (luc+) showed a posttranslationally-generated bioluminescence burst upon entry into the stationary phase. In this paper, we studied the mechanism underpinning this burst by using a series of "Keio" gene deletion strains. When luc+ driven by the lac gene promoter (lacp::luc+) was introduced into a group of Keio strains, the resulting reporter strains showed significantly altered timing and/or sizes of the burst. Remarkably, a reporter strain that lacked phosphoglucose isomerase (PGI), which catalyzes the second step of glycolysis, showed no burst, while the onset of the stationary phase of this strain was the same as that of the wild-type (WT) reporter strain. Consistently, the WT reporter strain showed no burst, when grown on arabinose or xylose instead of glucose as the carbon source. These results suggest that a process in carbohydrate metabolism is involved in the mechanism of generation of the burst. We measured temporal changes in intracellular NADPH concentrations but could not detect a significant increase or decrease relative to the occurrence of the burst. Functional implications and possible applications of the burst are discussed.
巻・号 532(2)
ページ 185-189
公開日 2020-11-5
DOI 10.1016/j.bbrc.2020.08.016
PII S0006-291X(20)31561-8
PMID 32859379
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism Benzothiazoles / pharmacology Carbohydrate Metabolism* / drug effects Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli / growth & development Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Gene Deletion Genes, Reporter Glucose-6-Phosphate Isomerase / genetics Glucose-6-Phosphate Isomerase / metabolism Glycolysis / physiology Luciferases / genetics Luciferases / metabolism Luminescent Measurements Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism NADP / metabolism
IF 2.985
リソース情報
原核生物(大腸菌)