RRC ID 66198
著者 Kamide T, Takusagawa S, Tanaka N, Ogasawara Y, Kawano Y, Ohtsu I, Satoh Y, Dairi T.
タイトル High Production of Ergothioneine in Escherichia coli using the Sulfoxide Synthase from Methylobacterium strains.
ジャーナル J Agric Food Chem
Abstract We previously constructed a heterologous production system for ergothioneine (ERG) in Escherichia coli using five ERG biosynthesis genes (egtABCDE) from Mycobacterium smegmatis. However, significant amounts of hercynine (HER), an intermediate of ERG, as ERG were accumulated, suggesting that the reaction of EgtB catalyzing the attachment of γ-glutamylcysteine (γGC) to HER to yield hercynyl-γ-glutamylcysteine sulfoxide was a bottleneck. In this study, we searched for other EgtBs and found many egtB orthologs in diverse microorganisms. Among these, Methylobacterium strains possessed EgtBs that catalyze the direct conversion of HER into hercynylcysteine sulfoxide with l-cysteine (l-Cys) as a sulfur donor, in a manner similar to those of acidobacterial CthEgtB and fungal Egt1. An in vitro study with recombinant EgtBs from Methylobacterium brachiatum and Methylobacterium pseudosasicola clearly showed that both enzymes accepted l-Cys but not γGC. We reconstituted the ERG production system in E. coli with egtB from M. pseudosasicola; ERG productivity reached 657 mg L-1.
巻・号 68(23)
ページ 6390-6394
公開日 2020-6-10
DOI 10.1021/acs.jafc.0c01846
PMID 32436380
MeSH Bacterial Proteins / genetics* Bacterial Proteins / metabolism Betaine / analogs & derivatives Betaine / metabolism Biosynthetic Pathways Dipeptides / metabolism Ergothioneine / biosynthesis Escherichia coli / genetics* Escherichia coli / metabolism* Histidine / analogs & derivatives Histidine / metabolism Metabolic Engineering Methylobacterium / enzymology* Methylobacterium / genetics Sulfoxides / metabolism*
IF 4.192
リソース情報
原核生物(大腸菌)