論文 - 詳細
| RRC ID | 66198 |
|---|---|
| 著者 | Kamide T, Takusagawa S, Tanaka N, Ogasawara Y, Kawano Y, Ohtsu I, Satoh Y, Dairi T. |
| タイトル | High Production of Ergothioneine in Escherichia coli using the Sulfoxide Synthase from Methylobacterium strains. |
| ジャーナル | J Agric Food Chem |
| Abstract |
We previously constructed a heterologous production system for ergothioneine (ERG) in Escherichia coli using five ERG biosynthesis genes (egtABCDE) from Mycobacterium smegmatis. However, significant amounts of hercynine (HER), an intermediate of ERG, as ERG were accumulated, suggesting that the reaction of EgtB catalyzing the attachment of γ-glutamylcysteine (γGC) to HER to yield hercynyl-γ-glutamylcysteine sulfoxide was a bottleneck. In this study, we searched for other EgtBs and found many egtB orthologs in diverse microorganisms. Among these, Methylobacterium strains possessed EgtBs that catalyze the direct conversion of HER into hercynylcysteine sulfoxide with l-cysteine (l-Cys) as a sulfur donor, in a manner similar to those of acidobacterial CthEgtB and fungal Egt1. An in vitro study with recombinant EgtBs from Methylobacterium brachiatum and Methylobacterium pseudosasicola clearly showed that both enzymes accepted l-Cys but not γGC. We reconstituted the ERG production system in E. coli with egtB from M. pseudosasicola; ERG productivity reached 657 mg L-1. |
| 巻・号 | 68(23) |
| ページ | 6390-6394 |
| 公開日 | 2020-6-10 |
| DOI | 10.1021/acs.jafc.0c01846 |
| PMID | 32436380 |
| MeSH | Bacterial Proteins / genetics* Bacterial Proteins / metabolism Betaine / analogs & derivatives Betaine / metabolism Biosynthetic Pathways Dipeptides / metabolism Ergothioneine / biosynthesis Escherichia coli / genetics* Escherichia coli / metabolism* Histidine / analogs & derivatives Histidine / metabolism Metabolic Engineering Methylobacterium / enzymology* Methylobacterium / genetics Sulfoxides / metabolism* |
| IF | 4.192 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |