論文 - 詳細
| RRC ID | 66200 |
|---|---|
| 著者 | Miller TE, Beneyton T, Schwander T, Diehl C, Girault M, McLean R, Chotel T, Claus P, Cortina NS, Baret JC, Erb TJ. |
| タイトル | Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts. |
| ジャーナル | Science |
| Abstract |
Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts and mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. These droplets can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time. We demonstrate how these microdroplets can be programmed and controlled by adjusting internal compositions and by using light as an external trigger. We showcase the capability of our platform by integrating the crotonyl-coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle, a synthetic network for carbon dioxide conversion, to create an artificial photosynthetic system that interfaces the natural and the synthetic biological worlds. |
| 巻・号 | 368(6491) |
| ページ | 649-654 |
| 公開日 | 2020-5-8 |
| DOI | 10.1126/science.aaz6802 |
| PII | 368/6491/649 |
| PMID | 32381722 |
| PMC | PMC7610767 |
| MeSH | Acyl Coenzyme A Biocatalysis Biomimetics Carbon Cycle Carbon Dioxide / metabolism* Chloroplasts / metabolism* Chloroplasts / radiation effects* Light Photosynthesis / radiation effects Spinacia oleracea |
| IF | 41.846 |
| リソース情報 | |
| 原核生物(大腸菌) | |