論文 - 詳細
| RRC ID | 66202 |
|---|---|
| 著者 | Steunou AS, Bourbon ML, Babot M, Durand A, Liotenberg S, Yamaichi Y, Ouchane S. |
| タイトル | Increasing the copper sensitivity of microorganisms by restricting iron supply, a strategy for bio-management practices. |
| ジャーナル | Microb Biotechnol |
| Abstract |
Pollution by copper (Cu2+ ) extensively used as antimicrobial in agriculture and farming represents a threat to the environment and human health. Finding ways to make microorganisms sensitive to lower metal concentrations could help decreasing the use of Cu2+ in agriculture. In this respect, we showed that limiting iron (Fe) uptake makes bacteria much more susceptible to Cu2+ or Cd2+ poisoning. Using efflux mutants of the purple bacterium Rubrivivax gelatinosus, we showed that Cu+ and Cd2+ resistance relies on the expression of the Fur-regulated FbpABC and Ftr iron transporters. To support this conclusion, inactivation of these Fe-importers in the Cu+ or Cd2+ -ATPase efflux mutants gave rise to hypersensitivity towards these ions. Moreover, in metal overloaded cells the expression of FbpA, the periplasmic iron-binding component of the ferric ion transport FbpABC system was induced, suggesting that cells perceived an 'iron-starvation' situation and responded to it by inducing Fe-importers. In this context, the Fe-Sod activity increased in response to Fe homoeostasis dysregulation. Similar results were obtained for Vibrio cholerae and Escherichia coli, suggesting that perturbation of Fe-homoeostasis by metal excess appeared as an adaptive response commonly used by a variety of bacteria. The presented data support a model in which metal excess induces Fe-uptake to support [4Fe-4S] synthesis and thereby induce ROS detoxification system. |
| 巻・号 | 13(5) |
| ページ | 1530-1545 |
| 公開日 | 2020-9-1 |
| DOI | 10.1111/1751-7915.13590 |
| PMID | 32558275 |
| PMC | PMC7415376 |
| MeSH | Burkholderiales* Copper* / toxicity Escherichia coli / genetics Humans Iron |
| IF | 5.328 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection JW3879-KC JW1648-KC |