| RRC ID |
66202
|
| 著者 |
Steunou AS, Bourbon ML, Babot M, Durand A, Liotenberg S, Yamaichi Y, Ouchane S.
|
| タイトル |
Increasing the copper sensitivity of microorganisms by restricting iron supply, a strategy for bio-management practices.
|
| ジャーナル |
Microb Biotechnol
|
| Abstract |
Pollution by copper (Cu2+ ) extensively used as antimicrobial in agriculture and farming represents a threat to the environment and human health. Finding ways to make microorganisms sensitive to lower metal concentrations could help decreasing the use of Cu2+ in agriculture. In this respect, we showed that limiting iron (Fe) uptake makes bacteria much more susceptible to Cu2+ or Cd2+ poisoning. Using efflux mutants of the purple bacterium Rubrivivax gelatinosus, we showed that Cu+ and Cd2+ resistance relies on the expression of the Fur-regulated FbpABC and Ftr iron transporters. To support this conclusion, inactivation of these Fe-importers in the Cu+ or Cd2+ -ATPase efflux mutants gave rise to hypersensitivity towards these ions. Moreover, in metal overloaded cells the expression of FbpA, the periplasmic iron-binding component of the ferric ion transport FbpABC system was induced, suggesting that cells perceived an 'iron-starvation' situation and responded to it by inducing Fe-importers. In this context, the Fe-Sod activity increased in response to Fe homoeostasis dysregulation. Similar results were obtained for Vibrio cholerae and Escherichia coli, suggesting that perturbation of Fe-homoeostasis by metal excess appeared as an adaptive response commonly used by a variety of bacteria. The presented data support a model in which metal excess induces Fe-uptake to support [4Fe-4S] synthesis and thereby induce ROS detoxification system.
|
| 巻・号 |
13(5)
|
| ページ |
1530-1545
|
| 公開日 |
2020-9-1
|
| DOI |
10.1111/1751-7915.13590
|
| PMID |
32558275
|
| PMC |
PMC7415376
|
| MeSH |
Burkholderiales*
Copper* / toxicity
Escherichia coli / genetics
Humans
Iron
|
| IF |
5.328
|
| リソース情報 |
| 原核生物(大腸菌) |
Keio collection
JW3879-KC
JW1648-KC |
