| RRC ID |
66204
|
| 著者 |
Liu L, Kurokawa M, Nagai M, Seno S, Ying BW.
|
| タイトル |
Correlated chromosomal periodicities according to the growth rate and gene expression.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Linking genetic information to population fitness is crucial to understanding living organisms. Despite the abundant knowledge of the genetic contribution to growth, the overall patterns/features connecting genes, their expression, and growth remain unclear. To reveal the quantitative and direct connections, systematic growth assays of single-gene knockout Escherichia coli strains under both rich and poor nutritional conditions were performed; subsequently, the resultant growth rates were associated with the original expression levels of the knockout genes in the parental genome. Comparative analysis of growth and the transcriptome identified not only the nutritionally differentiated fitness cost genes but also a significant correlation between the growth rates of the single-gene knockout strains and the original expression levels of these knockout genes in the parental strain, regardless of the nutritional variation. In addition, the coordinated chromosomal periodicities of the wild-type transcriptome and the growth rates of the strains lacking the corresponding genes were observed. The common six-period periodicity was somehow attributed to the essential genes, although the underlying mechanism remains to be addressed. The correlated chromosomal periodicities associated with the gene expression-growth dataset were highly valuable for bacterial growth prediction and discovering the working principles governing minimal genetic information.
|
| 巻・号 |
10(1)
|
| ページ |
15531
|
| 公開日 |
2020-9-23
|
| DOI |
10.1038/s41598-020-72389-6
|
| PII |
10.1038/s41598-020-72389-6
|
| PMID |
32968121
|
| PMC |
PMC7511328
|
| MeSH |
Chromosomes, Bacterial / genetics*
Escherichia coli / genetics*
Gene Expression*
Gene Expression Regulation, Bacterial / genetics
Gene Knockout Techniques
Periodicity
|
| IF |
3.998
|
| リソース情報 |
| 原核生物(大腸菌) |
Keio collection |
