Reference - Detail
| RRC ID | 66214 |
|---|---|
| Author | Hans S, Haby B, Krausch N, Barz T, Neubauer P, Cruz-Bournazou MN. |
| Title | Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations. |
| Journal | Bioengineering (Basel) |
| Abstract |
In bioprocess development, the host and the genetic construct for a new biomanufacturing process are selected in the early developmental stages. This decision, made at the screening scale with very limited information about the performance in larger reactors, has a major influence on the efficiency of the final process. To overcome this, scale-down approaches during screenings that show the real cell factory performance at industrial-like conditions are essential. We present a fully automated robotic facility with 24 parallel mini-bioreactors that is operated by a model-based adaptive input design framework for the characterization of clone libraries under scale-down conditions. The cultivation operation strategies are computed and continuously refined based on a macro-kinetic growth model that is continuously re-fitted to the available experimental data. The added value of the approach is demonstrated with 24 parallel fed-batch cultivations in a mini-bioreactor system with eight different Escherichia coli strains in triplicate. The 24 fed-batch cultivations were run under the desired conditions, generating sufficient information to define the fastest-growing strain in an environment with oscillating glucose concentrations similar to industrial-scale bioreactors. |
| Volume | 7(4) |
| Published | 2020-11-11 |
| DOI | 10.3390/bioengineering7040145 |
| PII | bioengineering7040145 |
| PMID | 33187191 |
| PMC | PMC7711848 |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | JW0554-KC JW3975-KC JW1907-KC JW2076-KC JW2082-KC JW2943-KC |