論文 - 詳細
| RRC ID | 6629 |
|---|---|
| 著者 | Yoshizaki T, Motomura W, Tanno S, Kumei S, Yoshizaki Y, Tanno S, Okumura T. |
| タイトル | Thiazolidinediones enhance vascular endothelial growth factor expression and induce cell growth inhibition in non-small-cell lung cancer cells. |
| ジャーナル | J Exp Clin Cancer Res |
| Abstract |
BACKGROUND:It is known that thiazolidinediones are involved in regulating the expression of various genes, including the vascular endothelial growth factor (VEGF) gene via peroxisome proliferator-activated receptor gamma (PPARgamma); VEGF is a prognostic biomarker for non-small-cell lung cancer (NSCLC). METHODS:In this study, we investigated the effects of troglitazone and ciglitazone on the mRNA expression of VEGF and its receptors in human NSCLC cell lines, RERF-LC-AI, SK-MES-1, PC-14, and A549. These mRNA expressions were evaluated by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. We also studied the effect of Je-11, a VEGF inhibitor, on the growth of these cells. RESULTS:In NSCLC cells, thiazolidinediones increased the mRNA expression of VEGF and neuropilin-1, but not that of other receptors such as fms-like tyrosine kinase and kinase insert domain receptor-1. Furthermore, the PPARgamma antagonist GW9662 completely reversed this thiazolidinedione-induced increase in VEGF expression. Furthermore, the addition of VEGF inhibitors into the culture medium resulted in the reversal of thiazolidinedione-induced growth inhibition. CONCLUSIONS:Our results indicated that thiazolidinediones enhance VEGF and neuropilin-1 expression and induce the inhibition of cell growth. We propose the existence of a pathway for arresting cell growth that involves the interaction of thiazolidinedione-induced VEGF and neuropilin-1 in NSCLC. |
| 巻・号 | 29(1) |
| ページ | 22 |
| 公開日 | 2010-3-10 |
| DOI | 10.1186/1756-9966-29-22 |
| PII | 1756-9966-29-22 |
| PMID | 20214829 |
| PMC | PMC2851675 |
| MeSH | Carcinoma, Non-Small-Cell Lung / embryology* Cell Line, Tumor Cell Proliferation Culture Media / pharmacology Humans Hypoglycemic Agents / pharmacology Lung Neoplasms / metabolism* Neuropilin-1 / biosynthesis PPAR gamma / metabolism Phosphorylation RNA, Messenger / metabolism Thiazolidinediones / pharmacology* Vascular Endothelial Growth Factor A / biosynthesis* |
| IF | 7.068 |
| 引用数 | 11 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) PC-14(RCB0446) |