| RRC ID |
6631
|
| 著者 |
Amano M, Yamaguchi M, Takegawa Y, Yamashita T, Terashima M, Furukawa J, Miura Y, Shinohara Y, Iwasaki N, Minami A, Nishimura S.
|
| タイトル |
Threshold in stage-specific embryonic glycotypes uncovered by a full portrait of dynamic N-glycan expression during cell differentiation.
|
| ジャーナル |
Mol Cell Proteomics
|
| Abstract |
Although various glycoforms appear to participate independently in multiple molecular interactions in cellular adhesion that contribute to embryogenesis and organogenesis, a full portrait of the glycome diversity and the effect of the structural variations of cellular glycoforms on individual cell stages in proliferation and differentiation remain unclear. Here we describe a novel concept for the characterization of dynamic glycoform alteration during cell differentiation by means of "glycoblotting-based cellular glycomics," the only method allowing for rapid and quantitative glycan analysis. We demonstrated that processes of dynamic cellular differentiation of mouse embryonic carcinoma cells, P19CL6 and P19C6, and mouse embryonic stem cells into cardiomyocytes or neural cells can be monitored and characterized quantitatively by profiling entire N-glycan structures of total cell glycoproteins. Whole N-glycans enriched and identified by the glycoblotting method (67 glycans for P19CL6, 75 glycans for P19C6, and 72 glycans for embryonic stem cells) were profiled and bar-coded quantitatively with respect to the ratio of subgroups composed of characteristic glycoforms, namely glycotypes.
|
| 巻・号 |
9(3)
|
| ページ |
523-37
|
| 公開日 |
2010-3-1
|
| DOI |
10.1074/mcp.M900559-MCP200
|
| PII |
S1535-9476(20)30830-6
|
| PMID |
20008832
|
| PMC |
PMC2849713
|
| MeSH |
Animals
Cell Differentiation*
Cell Line
Embryonal Carcinoma Stem Cells / chemistry*
Embryonal Carcinoma Stem Cells / cytology
Embryonal Carcinoma Stem Cells / metabolism
Embryonic Development
Embryonic Stem Cells / chemistry*
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism
Glycomics / methods
Glycoproteins / analysis*
Glycoproteins / metabolism
Mice
Myocytes, Cardiac / chemistry*
Myocytes, Cardiac / cytology
Myocytes, Cardiac / metabolism
Neurons / chemistry*
Neurons / cytology
Neurons / metabolism
Organogenesis
Polysaccharides / analysis*
|
| IF |
4.87
|
| 引用数 |
39
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| ヒト・動物細胞 |
|
