| RRC ID |
66323
|
| 著者 |
Udomsopagit T, Miwa A, Seki M, Shimbori E, Kadota Y, Tochio T, Sonoyama K.
|
| タイトル |
Intestinal microbiota transplantation reveals the role of microbiota in dietary regulation of RegIIIβ and RegIIIγ expression in mouse intestine.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
RegIIIβ and RegIIIγ are antimicrobial peptides expressed in intestinal epithelial cells. Expression of these peptides is reportedly decreased by high-fat diet (HFD) and increased by indigestible oligosaccharides in mice. Clearly, these dietary regimens change the structure of intestinal microbiota. We employed an intestinal microbiota transplantation (IMT) to test whether diet-induced changes in the expression of these peptides are mediated by gut microbiota. C57BL/6J mice were fed either a normal-fat diet (NFD), a HFD, or a NFD supplemented with or without 1-kestose (KES), an indigestible oligosaccharide. Ileal RegIIIβ and RegIIIγ mRNA levels were lower in mice receiving IMT from HFD-fed mice than in those receiving NFD-fed mice and higher in mice receiving IMT from KES-supplemented mice than in those receiving the mice without KES supplementation. Western blot analysis showed that serum RegIIIβ levels changed in parallel with the ileal mRNA levels. We propose that HFD- and KES-induced changes in the ileal RegIIIβ and RegIIIγ expression and in the circulating RegIIIβ levels are mediated, at least in part, by intestinal microbiota.
|
| 巻・号 |
529(1)
|
| ページ |
64-69
|
| 公開日 |
2020-8-13
|
| DOI |
10.1016/j.bbrc.2020.05.150
|
| PII |
S0006-291X(20)31085-8
|
| PMID |
32560820
|
| MeSH |
Animals
Diet
Diet, High-Fat
Fecal Microbiota Transplantation*
Gastrointestinal Microbiome*
Ileum / metabolism
Interleukin-22
Interleukins / genetics
Interleukins / metabolism
Male
Mice
Mice, Inbred C57BL
Pancreatitis-Associated Proteins / blood*
Pancreatitis-Associated Proteins / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Trisaccharides / administration & dosage
|
| リソース情報 |
| 一般微生物 |
JCM1395
JCM11019
JCM1190 |
