RRC ID 6635
Author Iwasaki J, Hata T, Hishikawa S, Fujimoto Y, Uemoto S, Murakami T, Kobayashi E.
Title Use of rat segmental intestine for fetal pancreatic transplantation.
Journal Microsurgery
Abstract It is thought that the small intestine may provide a scaffold for pancreas regeneration. Herein, we investigated whether fetal pancreatic tissue could be transplanted into the segmental intestine in rats. Fetal pancreases from firefly luciferase transgenic Lewis rat embryos (embryonic day 14.5 and 15.5) were transplanted into streptozotocin (STZ)-induced diabetic wild-type Lewis rats. As a scaffold for pancreatic development, rat small intestinal segments were utilized after the removal of mucosa, and fetal pancreases were grafted into the luminal surface through the stoma. We also transplanted fetal pancreases into the omentum. The survival of transplanted fetal pancreases was monitored by luciferase-derived photons and blood glucose levels. Transplanted fetal pancreas-derived photons were stable for 28 days, suggesting that transplanted fetal pancreatic tissues survived and that their intestinal blood supply was maintained.
Volume 30(4)
Pages 296-301
Published 2010-5-1
DOI 10.1002/micr.20771
PMID 20309851
MeSH Animals Blood Glucose / analysis Blood Glucose / metabolism* Diabetes Mellitus, Experimental / surgery* Disease Models, Animal Female Fetal Tissue Transplantation / adverse effects Fetal Tissue Transplantation / methods* Graft Rejection Graft Survival Jejunum / pathology Jejunum / surgery* Male Pancreas Transplantation / adverse effects Pancreas Transplantation / methods* Random Allocation Rats Rats, Inbred Lew Rats, Transgenic Risk Factors Statistics, Nonparametric Survival Rate
IF 1.996
Times Cited 1
WOS Category SURGERY
Resource
Rats LEW-Tg(Gt(ROSA)26Sor-luc)11Jmsk(strainID=649) LEW-Tg(Gt(ROSA)26Sor-luc)22Jmsk