論文 - 詳細
| RRC ID | 66404 |
|---|---|
| 著者 | Singh RP, Rajarammohan S, Thakur R, Hassan M. |
| タイトル | Linear and branched β-Glucans degrading enzymes from versatile Bacteroides uniformis JCM 13288T and their roles in cooperation with gut bacteria. |
| ジャーナル | Gut Microbes |
| Abstract |
β-glucans are the dietary nutrients present in oats, barley, algae, and mushrooms. The macromolecules are well known for their immune-modulatory activity; however, how the human gut bacteria digest them is vaguely understood. In this study, Bacteroides uniformis JCM 13288 T was found to grow on laminarin, pustulan, and porphyran. We sequenced the genome of the strain, which was about 5.05 megabase pairs and contained 4868 protein-coding genes. On the basis of growth patterns of the bacterium, two putative polysaccharide utilization loci for β-glucans were identified from the genome, and associated four putative genes were cloned, expressed, purified, and characterized. Three glycoside hydrolases (GHs) that were endo-acting enzymes (BuGH16, BuGH30, and BuGH158), and one which was an exo-acting (BuGH3) enzyme. The BuGH3, BuGH16, and BuGH158 can cleave linear exo/endo- β- 1-3 linkages while BuGH30 can digest endo- β- 1-6 linkages. BuGH30 and BuGH158 were further explored for their roles in digesting β- glucans and generation of oligosaccharides, respectively. The BuGH30 predominately found to cleave long chain β- 1-6 linked glucans, and obtained final product was gentiobiose. The BuGH158 used for producing oligosaccharides varying from degree of polymerization 2 to 7 from soluble curdlan. We demonstrated that these oligosaccharides can be utilized by gut bacteria, which either did not grow or poorly grew on laminarin. Thus, B. uniformis JCM 13288 T is not only capable of utilizing β-glucans but also shares these glycans with human gut bacteria for potentially maintaining the gut microbial homeostasis. |
| 巻・号 | 12(1) |
| ページ | 1-18 |
| 公開日 | 2020-11-9 |
| DOI | 10.1080/19490976.2020.1826761 |
| PMID | 33043794 |
| PMC | PMC7553746 |
| MeSH | Bacteroides / enzymology Bacteroides / growth & development Bacteroides / metabolism* Carbohydrate Conformation Gastrointestinal Microbiome* Genetic Loci Genome, Bacterial Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism* Gram-Positive Bacteria / genetics Gram-Positive Bacteria / growth & development Gram-Positive Bacteria / metabolism* Microbial Interactions Oligosaccharides / metabolism Polysaccharides / metabolism beta-Glucans / chemistry beta-Glucans / metabolism* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM13288 JCM1471 JCM15917 JCM1200 JCM1275 JCM13470 |