論文 - 詳細
| RRC ID | 6658 |
|---|---|
| 著者 | Bogdanovic A, Bruckert F, Morio T, Satre M. |
| タイトル | A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion. |
| ジャーナル | J Biol Chem |
| Abstract |
Endo-phagocytic activity is prominent in Dictyostelium discoideum and makes it a good model organism to study the molecular organization of membrane traffic in this pathway. We have identified a syntaxin 7 homologue (26% identity and 54% similarity to human syntaxin 7) in Dictyostelium cDNA and genomic data banks. In addition to the Habc and H3 helices and the C-terminal transmembrane domain characteristic of syntaxins, this protein contains a repetitive N-terminal extension of 68 amino acids. We first showed that Dictyostelium syntaxin 7 was able to form a complex with N-ethylmaleimide-sensitive fusion protein and alpha- and gamma-soluble N-ethylmaleimide-sensitive fusion protein attachment protein. Its intracellular localization was then studied by cell fractionation techniques and magnetic purification of the endocytic compartments. Most of D. discoideum syntaxin 7 is contained in endosomes. Finally, an in vitro endosome homotypic fusion assay (Laurent, O., Bruckert, F., Adessi, C., and Satre, M. (1998) J. Biol. Chem. 273, 793-799) was used to study a possible role for syntaxin 7 in this process. Purified anti-syntaxin 7 antibodies and a recombinant soluble fragment of syntaxin 7 both strongly inhibited fusion activity, indicating that this protein was necessary for endosome-endosome fusion. These results demonstrate the importance of this syntaxin 7 homologue in the early phases of Dictyostelium endo-phagocytic pathway. |
| 巻・号 | 275(47) |
| ページ | 36691-7 |
| 公開日 | 2000-11-24 |
| DOI | 10.1074/jbc.M006710200 |
| PII | S0021-9258(20)88569-X |
| PMID | 10978342 |
| MeSH | Amino Acid Sequence Animals Blotting, Western Centrifugation, Density Gradient Dictyostelium / chemistry* Endosomes / chemistry* Humans Membrane Proteins / chemistry Membrane Proteins / isolation & purification* Molecular Sequence Data Qa-SNARE Proteins Sequence Homology, Amino Acid |
| IF | 4.238 |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 各媒体での言及数の合計 | 0 |
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