RRC ID |
6663
|
Author |
Morio T, Yasukawa H, Urushihara H, Saito T, Ochiai H, Takeuchi I, Maeda M, Tanaka Y.
|
Title |
FebA: a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum.
|
Journal |
Biochim Biophys Acta
|
Abstract |
We have identified a gene encoding a eukaryotic initiation factor 4E-binding protein (4E-BP) in the EST database of the Dictyostelium cDNA project. The Dictyostelium 4E-BP, designated febA (four e-binding), showed significant similarity to mammalian 4E-BPs. Northern blot analysis revealed that febA was expressed at a high level in the vegetative growth phase but the level of expression decreased during late development. The gene was shown to be non-essential since disruption of the gene had no severe effect; the null mutant proliferated normally and formed normal fruiting bodies. However, strains overexpressing the gene could not be established, suggesting that an excess of FebA protein may have a lethal effect on the cells.
|
Volume |
1519(1-2)
|
Pages |
65-9
|
Published |
2001-5-28
|
DOI |
10.1016/s0167-4781(01)00219-6
|
PII |
S0167-4781(01)00219-6
|
PMID |
11406272
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins / genetics*
DNA, Complementary / chemistry
DNA, Complementary / isolation & purification
Dictyostelium / genetics*
Genetic Vectors
Molecular Sequence Data
Phosphoproteins*
Sequence Alignment
|
IF |
3.411
|
Times Cited |
0
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOPHYSICS
|
Resource |
Cellular slime molds |
G00593 |