RRC ID 6664
Author Okuwa T, Morlo T, Saito T, Masamune Y, Yasukawa H.
Title Complete sequences and expression kinetics of racG, racH, racI and racJ genes in Dictyostelium discoideum.
Journal Biol Pharm Bull
Abstract We sequenced and characterized the expression patterns of the genes (racG, racH, racI and racJ) in the Rho-family. The nucleotide sequences of these genes suggest that racI would be a pseudogene, while the other genes are likely to encode typical Rac proteins which contain either GTP-binding domain or CAAX prenylation motif as observed in other members of the family. The Northern blot analyses show that the expression patterns of these genes are distinctively regulated during development. The racG gene is expressed at almost the same level from the vegetative to the slug stage, but the amount of its transcript gradually decreases after culmination. Expression of the racJ gene is undetectable at the vegetative stage, becomes observable at the mound stage, reaches a peak at the slug stage and then suddenly disappears in the culmination stage. The racH gene is expressed in two forms of transcripts, both of which are undetectable at the vegetatively growing stage but abruptly increase in amount after starvation. Southern blot hybridization analysis demonstrates that these transcripts were derived from a single copy of the gene. Such distinct kinetics of the expression patterns suggests that these genes would have unique roles in Dictyostelium development.
Volume 24(1)
Pages 84-7
Published 2001-1-1
DOI 10.1248/bpb.24.84
PMID 11201251
MeSH Amino Acid Sequence Animals Base Sequence DNA Dictyostelium / genetics* Dictyostelium / growth & development Gene Expression Regulation, Developmental Kinetics Molecular Sequence Data Sequence Homology, Amino Acid rac GTP-Binding Proteins / genetics*
IF 1.863
Times Cited 3
Cellular slime molds G03171 G02110 G20257 G02029 G02119 G03213 G01311 G00891 G01272 G00743 G02434 G00666 G00858