RRC ID 6670
Author Gojković Z, Sandrini MP, Piskur J.
Title Eukaryotic beta-alanine synthases are functionally related but have a high degree of structural diversity.
Journal Genetics
Abstract beta-Alanine synthase (EC, which catalyzes the final step of pyrimidine catabolism, has only been characterized in mammals. A Saccharomyces kluyveri pyd3 mutant that is unable to grow on N-carbamyl-beta-alanine as the sole nitrogen source and exhibits diminished beta-alanine synthase activity was used to clone analogous genes from different eukaryotes. Putative PYD3 sequences from the yeast S. kluyveri, the slime mold Dictyostelium discoideum, and the fruit fly Drosophila melanogaster complemented the pyd3 defect. When the S. kluyveri PYD3 gene was expressed in S. cerevisiae, which has no pyrimidine catabolic pathway, it enabled growth on N-carbamyl-beta-alanine as the sole nitrogen source. The D. discoideum and D. melanogaster PYD3 gene products are similar to mammalian beta-alanine synthases. In contrast, the S. kluyveri protein is quite different from these and more similar to bacterial N-carbamyl amidohydrolases. All three beta-alanine synthases are to some degree related to various aspartate transcarbamylases, which catalyze the second step of the de novo pyrimidine biosynthetic pathway. PYD3 expression in yeast seems to be inducible by dihydrouracil and N-carbamyl-beta-alanine, but not by uracil. This work establishes S. kluyveri as a model organism for studying pyrimidine degradation and beta-alanine production in eukaryotes.
Volume 158(3)
Pages 999-1011
Published 2001-7-1
DOI 10.1093/genetics/158.3.999
PMID 11454750
PMC PMC1461717
MeSH Amidohydrolases / genetics Amidohydrolases / metabolism* Amino Acid Sequence Animals Base Sequence Cloning, Molecular Dictyostelium / enzymology Drosophila melanogaster / enzymology Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Protein Conformation RNA, Messenger / genetics Saccharomyces / enzymology Saccharomyces / genetics Saccharomyces / growth & development Sequence Homology, Amino Acid
IF 4.015
Times Cited 43
Cellular slime molds G02262